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pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

Plasmid

#122067

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Publication

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW YPET-T2A-mTurquoise2

Plasmid

#118450

Purpose

Produces equimolar expression of YPET and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

YPET-T2A-mTurquoise2

Expression

Yeast

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCuGFP-SpAtg17(416)

Plasmid

#122080

Purpose

Expressing S. pombe GFP-Atg17

Depositor

Daniel Klionsky

Publication

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG17 (atg17 Fission Yeast)

Tags

GFP

Expression

Yeast

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pESC/CURT_fluoB

Plasmid

#117997

Purpose

Expression of CURT_fluoB controlled by the Gal1 promoter

Depositor

Mathias Pribil

Publication

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_fluoB

Use

Yeast/bacteria binary vector

Expression

Yeast

Promoter

Gal1

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomSua1

Plasmid

#107446

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Publication

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sua1

Tags

V5-His

Expression

Yeast

Mutation

See depositor comments below.

Promoter

GAL1

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-MCP-2yeGFP(405)

Plasmid

#121419

Purpose

Expressing MCP-2yeGFP

Depositor

Daniel Klionsky

Publication

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

CUP1p-MCP-2yeGFP

Expression

Yeast

Promoter

CUP1 promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mkok-T2A-mTurquoise2

Plasmid

#118428

Purpose

Produces equimolar expression of mKokappa and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mkok-T2A-mTq2

Expression

Yeast

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17

Plasmid

#121386

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Publication

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Promoter

ADH1 promoter

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1675

Plasmid

#112049

Purpose

CEN/ARS plasmid for GAL-inducible TSC13-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Publication

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

TSC13

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCHKU34.1-2.2

Plasmid

#115534

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTurquoise2-T2A-mCherry

Plasmid

#118442

Purpose

Produces equimolar expression of yeast codon-optimized mTq2 and mCherry

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2-T2A-mCherry

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymYPET-T2A-mTurquoise2

Plasmid

#118444

Purpose

Produces equimolar expression of ymYPET and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET-T2A-mTurquoise2

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yomCherry-T2A-mTurquoise2

Plasmid

#118447

Purpose

Produces equimolar expression of yeast codon-optimized mCherry and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yomCherry-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ytdtomato-T2A-mTurquoise2

Plasmid

#118451

Purpose

Produces equimolar expression of yeast codon-optimized tdTomato and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdtomato-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mRuby2-T2A-mTurquoise2

Plasmid

#118429

Purpose

Produces equimolar expression of mRuby2 and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mRuby2-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW eCFP-T2A-mCherry

Plasmid

#118421

Purpose

Produces equimolar expression of eCFP and mCherry

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

eCFP-T2A-mCherry

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL18H_AB

Plasmid

#117827

Purpose

GoldenMOCS backbone for 1 TU in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL18H_AC

Plasmid

#117828

Purpose

GoldenMOCS backbone for 2 TU in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL68N_AE

Plasmid

#117832

Purpose

GoldenMOCS backbone for 4 TU in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL68N_AC

Plasmid

#117830

Purpose

GoldenMOCS backbone for 2 TU in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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