8,418 results
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Plasmid#113873Purposeexpression of Cas9 programming sgRNA8 and sgRNA7 targetting HXT10 and HXT9-11-12 respectivelyDepositorInsertsgRNA8-HXT10 sgRNA7-HXT9-11-12
UseCRISPRExpressionYeastAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUDR418
Plasmid#113875Purposeexpression of double Cas9 programming sgRNA11 targetting STL1DepositorInsertdoble sgRNA11-STL1
UseCRISPRExpressionYeastAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUDR295
Plasmid#113874Purposeexpression of Cas9 programming sgRNA2 and sgRNA1 targetting GAL2 and HXT4-1-5/ HXT3-6-7 respectivelyDepositorInsertsgRNA2 GAL2 sgRNA1-HXT4-1-5;HXT3-6-7
UseCRISPRExpressionYeastAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM3193
Plasmid#113390PurposeAlpha factor inducible Ty3 with his3AIDepositorInsertTy3-his3AI
Tagshis3AIExpressionYeastAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP4010
Plasmid#113336PurposeBeta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertsArtificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase
Tags3X-FLAG/8X-Gly linkerExpressionYeastAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKN3097
Plasmid#113392PurposeGalactose-inducible Ty3 with extra PPT- used for random bar code libraryDepositorInsertTy3
ExpressionYeastAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL10 - Brr2 del422 WT
Plasmid#90260Purposeprotein expressionDepositorInsertBRR2
TagsTAPExpressionYeastMutationdeleted first 422 amino acidsAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCB30
Plasmid#111910PurposeExpresses gRNA to target URA3 knockout locus and has KanMX markerDepositorInsertgRNA cassette targeting URA3 knockout locus
UseCRISPRExpressionBacterial and YeastPromoterSNR52pAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
sub2-1 pRS315
Plasmid#111301Purposesub2-1 in pRS315DepositorPublicationInsertSUB2
ExpressionYeastMutationsub2-1Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET5
Plasmid#107437PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET5 (MET5 Budding Yeast)
TagsV5-HisExpressionYeastMutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …PromoterGAL1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomSir1
Plasmid#107447PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS316-Cwc25
Plasmid#111343PurposeCwc25 in pRS316(Ura) vectorDepositorInsertCWC25
TagsnoneExpressionYeastAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_HIS3_I
Plasmid#109058Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.DepositorInsertHIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxExpressionBacterial and YeastPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_HIS3_II
Plasmid#109059Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.DepositorInsertHIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxExpressionBacterial and YeastPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_LEU2_I
Plasmid#109060Purposeyeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposesDepositorInsertLEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxExpressionBacterial and YeastPromoterLEU2Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A10-17
Plasmid#112826PurposepSMF2 with all S to A mutations in only repeats 10-17.DepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 1…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A2-9
Plasmid#112825PurposepSMF2 with all S to A mutations in only repeats 2-9.DepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 2…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A18-25
Plasmid#112827PurposepSMF2 with all S to A mutations in only repeats 18-25.DepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 1…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD8
Plasmid#112820PurposepSMF2 with 8 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303 prp8-101
Plasmid#111307Purposeprp8-101DepositorPublicationAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only