8,057 results

1. pSL12 - Brr2 del422 Q904E

   Plasmid

   #90262

   Purpose

   protein expression

   Depositor

   Christine Guthrie

   Article

   Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

   Insert

   BRR2
   

   Use

   Tags

   TAP

   Expression

   Yeast

   Mutation

   deleted first 422 amino acids and amino acid Q904E

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSL13 - Brr2 del422 R1107L

   Plasmid

   #90263

   Purpose

   protein expression

   Depositor

   Christine Guthrie

   Article

   Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

   Insert

   BRR2
   

   Use

   Tags

   TAP

   Expression

   Yeast

   Mutation

   deleted first 422 amino acids and amino acid R110…

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHES942

   Plasmid

   #90408

   Purpose

   Expresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pZ-SIC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Sub2-3 in pRS314

   Plasmid

   #90454

   Purpose

   yeast

   Depositor

   Christine Guthrie

   Article

   Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

   Insert

   SUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   sub2-3 mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Sub2-4 in pRS314

   Plasmid

   #90455

   Purpose

   yeast

   Depositor

   Christine Guthrie

   Article

   Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

   Insert

   SUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   sub2-4 mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Sub2-6 in pRS314

   Plasmid

   #90456

   Purpose

   yeast

   Depositor

   Christine Guthrie

   Article

   Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

   Insert

   SUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   sub2-6 mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L284F Cus2p

   Plasmid

   #73059

   Purpose

   S. cerevisiae Cus2p gene with L284F Mutation

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 Dec 2.

   Insert

   CUS2p (CUS2 Budding Yeast)
   

   Use

   Tags

   6x His tag fused to TEV cleavage site

   Expression

   Bacterial and Yeast

   Mutation

   Leucine 284 to Phenylalanine

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. D282N Cus2p

   Plasmid

   #73060

   Purpose

   S. cerevisiae Cus2p gene with D282N Mutation

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 Dec 2.

   Insert

   CUS2p (CUS2 Budding Yeast)
   

   Use

   Tags

   6x His tag fused to TEV cleavage site

   Expression

   Bacterial and Yeast

   Mutation

   Asp 282 to Asn

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAV10.F3.loxP

   Plasmid

   #63200

   Purpose

   pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAV10.K3.loxP

    Plasmid

    #63202

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV10.K6.loxP

    Plasmid

    #63203

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV10.K5.loxP

    Plasmid

    #63204

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV10.HO3.loxP

    Plasmid

    #63205

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAV10.HO5.loxP

    Plasmid

    #63206

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV10.HO6

    Plasmid

    #63211

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. brr2-Q904E pRS313

    Plasmid

    #111413

    Purpose

    brr2-Q904E (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q904E aa mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. brr2-R1107A pRS313

    Plasmid

    #111415

    Purpose

    brr2-R1107A (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R1107A aa mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. brr2-R1107M pRS313

    Plasmid

    #111417

    Purpose

    brr2-R1107M (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R1107M aa mutation (note, not an RP allele)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pV1338

    Plasmid

    #111443

    Purpose

    Solo vector pV1326 + sgScADE2

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgScADE2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. yFusionRed

    Plasmid

    #111916

    Purpose

    yeast optimized FusionRed

    Depositor

    Patrick Lajoie

    Article

    Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

    Insert

    yFusionRed
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHCMM1

    Plasmid

    #167452

    Purpose

    Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHCMM2

    Plasmid

    #167453

    Purpose

    Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PSEC63

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHCMM3

    Plasmid

    #167454

    Purpose

    A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PSEC63

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p414GPD-3xFLAG-Nop4 L306P

    Plasmid

    #169262

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306P

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 L306P (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    p.(Leu306Pro)

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p414GPD-3xFLAG-Nop4 dE5

    Plasmid

    #169263

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 delta E5 (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted region corresponding to human Exon 5

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p414GPD-3xFLAG-Nop4 dE8

    Plasmid

    #169264

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 delta E8 (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted region corresponding to human Exon 8

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p414GPD-3xFLAG-RBM28 WT

    Plasmid

    #169265

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WT

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 WT (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p414GPD-3xFLAG-RBM28 L351P

    Plasmid

    #169266

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351P

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 L351P (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    p.(Leu351Pro)

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p414GPD-3xFLAG-RBM28 dE5

    Plasmid

    #169267

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 delta E5 (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted Exon 5

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p414GPD-3xFLAG-RBM28 dE8

    Plasmid

    #169268

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 delta E8 (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted Exon 8

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. WT Ecm2

    Plasmid

    #169922

    Purpose

    Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Ecm2 1-143

    Plasmid

    #169923

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-143
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Ecm2 1-265

    Plasmid

    #169925

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-265
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Ecm2 1-325

    Plasmid

    #169926

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-325
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pILGFP4G

    Plasmid

    #104502

    Purpose

    Used to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    ScPGM2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pILGFP4K

    Plasmid

    #104506

    Purpose

    Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SpGAL1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pILGFP4O

    Plasmid

    #104510

    Purpose

    Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SpdGAL2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pILGFP4P

    Plasmid

    #104511

    Purpose

    Used to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SptGAL2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJW1668

    Plasmid

    #112042

    Purpose

    CEN/ARS plasmid for GAL-inducible FET3-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    FET3
    

    Use

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJW1669

    Plasmid

    #112043

    Purpose

    CEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    NSG2
    

    Use

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge