8,010 results

1. pAV0661

   Plasmid

   #133476

   Purpose

   Expression in fission yeast from promoter pact1, integrated at ade6 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV10.KH

   Plasmid

   #63208

   Purpose

   pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIplac211-Vph1-HaloTag

   Plasmid

   #140149

   Purpose

   Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-HaloTag

   Depositor

   Benjamin Glick

   Article

   Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

   Insert

   VPH1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIM691

   Plasmid

   #74641

   Purpose

   alias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Insert

   ymTagBFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-mTID-HA3-HIS3MX6

   Plasmid

   #227983

   Purpose

   To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   miniTurboID
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1

   Plasmid

   #58432

   Purpose

   Induces Citrine expression from a promoter containing 1 lexA box in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)1-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSP-G1

   Plasmid

   #64736

   Purpose

   Yeast expression plasmid

   Depositor

   Jens Nielsen

   Article

   Partow et al Yeast. 2010 Nov;27(11):955-64. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG426-GAL-Alpha-synuclein-H50Q

   Plasmid

   #181724

   Purpose

   Galactose inducible expression of Alpha-synuclein-H50Q

   Depositor

   Alejandro Chavez

   Insert

   Alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H50Q

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS315e_pGal-dCas9-PmCDA1

   Plasmid

   #79615

   Purpose

   Expresses dCas9-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL513

    Plasmid

    #169153

    Purpose

    Encodes OptoQ-INVRT1

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRR-AR-5Z

    Plasmid

    #23058

    Depositor

    Charles Miller

    Article

    Miller et al Plasmid. 2009 Dec 3. ():.

    Insert

    AR (AR Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pXZX501

    Plasmid

    #169455

    Purpose

    pRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassette

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    gRNA for MATa
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJZC533

    Plasmid

    #135435

    Purpose

    Yeast integrating plasmid for pTEF1-mCherry reporter gene

    Depositor

    Jesse Zalatan

    Article

    Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    pTEF1

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. bRA66

    Plasmid

    #100952

    Purpose

    GAL1 driven Cas9. gRNA can be cloned into BplI sites.

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYX233

    Plasmid

    #163762

    Purpose

    Empty vector for Gal-inducible yeast expression

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Type

    Empty backbone

    Use

    Tags

    Hemagglutinin (HA) tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKL05

    Plasmid

    #104429

    Purpose

    super Ecliptic pHluorin in yeast expression vector

    Depositor

    Joel Kralj

    Article

    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

    Insert

    Super Ecliptic pHluorin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pV1393

    Plasmid

    #111430

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SJZ7-KAN

    Plasmid

    #228260

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    kan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPM1 S.c.

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SJZ4-NAT

    Plasmid

    #228257

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    nat1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    FBA1 S.c.

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pILGFP5EC4

    Plasmid

    #185880

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pILGFP3AG4ARSd

    Plasmid

    #185876

    Purpose

    A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pILGFPE9

    Plasmid

    #83545

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVG122

    Plasmid

    #211765

    Purpose

    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    strongLOV
    

    Use

    For single-copy integration (if cut with pmei)

    Tags

    SV40 NLS and VP16

    Expression

    Yeast

    Mutation

    compared to EL222, strongLOV contains Glu84Asp mu…

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNA0306

    Plasmid

    #169452

    Purpose

    pRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmid

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1p

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYSD300

    Plasmid

    #212930

    Purpose

    Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    AGA1 (AGA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PHBA02

    Plasmid

    #83536

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. DdMCU

    Plasmid

    #67642

    Purpose

    DdMCU-Flag in pACT2 vector human codon optimised

    Depositor

    Vamsi Mootha

    Article

    Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    DdMCU
    

    Use

    Tags

    Flag tag with linker

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA6a-ura4MX6

    Plasmid

    #49184

    Purpose

    gene deletion in S. pombe

    Depositor

    Eishi Noguchi

    Article

    Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR

    Plasmid

    #24584

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMZ379

    Plasmid

    #74215

    Purpose

    Combined sgRNA/Cas9 vector with NatMX marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKT127-SEP

    Plasmid

    #41899

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Type

    Empty backbone

    Use

    Tags

    superecliptic GFP (SEP)

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p112A1NE

    Plasmid

    #85983

    Purpose

    For construction of cDNA expression library.

    Depositor

    Wolf Frommer

    Article

    Riesmeier et al EMBO J. 1992 Dec;11(13):4705-13.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Adh1

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. M4786 KanMX::hisG-URA3-HisG Disruptor Converter

    Plasmid

    #51690

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    hisG-URA3-hisG (URA3 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p416 25Q GAL

    Plasmid

    #1185

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-link-yomTagBFP-SpHis5

    Plasmid

    #44838

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mTagBFP with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTH733-2µ-RLuc/maxCFLuc

    Plasmid

    #40608

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ZJOM70

    Plasmid

    #133658

    Purpose

    Integration of ERG6-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ERG6-mCherry (ERG6 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRSCupSup35GFP

    Plasmid

    #1087

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

    Plasmid

    #24549

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge