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GAL FLAG72QDProGFP p306

Plasmid

#15833

Depositor

Susan Lindquist

Publication

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
pAG426GAL-ccdB-DsRed

Plasmid

#14371

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

DsRed

Expression

Yeast

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAG423GPD-ccdB-EGFP

Plasmid

#14198

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKT0178

Plasmid

#8744

Depositor

Kurt Thorn

Publication

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

tdimer2

Tags

Codon optimized linker

Expression

Yeast

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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3396 pBM272 Bax

Plasmid

#8770

Depositor

Stanley Korsmeyer

Publication

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bax (Bax Mouse)

Expression

Yeast

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pL2/GZ

Plasmid

#1282

Depositor

Susan Lindquist

Publication

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

GRE-lacZ

Tags

glucocorticoid response elemen-lacZ

Expression

Yeast

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
pBHM2404

Plasmid

#173441

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

Depositor

Hiten Madhani

Publication

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-NAT1

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYX233-b2delta-DHFR

Plasmid

#163760

Purpose

Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFR

Depositor

Johannes Herrmann

Publication

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

b2delta-DHFR (Dhfr Budding Yeast, Mouse)

Expression

Yeast

Mutation

Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

Promoter

GAL1

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNTI661 pRPR1(TetO)-sgRNA

Plasmid

#139475

Purpose

Vector for tet-regulated sgRNA expression in yeast

Depositor

Nicholas Ingolia

Publication

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Insert

single guide RNA scaffold

Use

CRISPR

Expression

Yeast

Promoter

pRPR1(TetO)

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ZJOM149

Plasmid

#133646

Purpose

Integration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

Depositor

Zhiping Xie

Publication

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-ELO3-mCherry (ELO3 Budding Yeast)

Tags

mCherry

Expression

Yeast

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRS306-PHIS3-GFP-μNS

Plasmid

#116935

Purpose

Yeast integrative vector for the expression of μNS

Depositor

Liam Holt

Publication

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

μNS

Tags

GFP

Expression

Yeast

Promoter

HIS3

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BB3cH_pGAP_23*_pPFK300_Cas9

Plasmid

#104912

Purpose

hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

Depositor

Brigitte Gasser

Publication

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Expression

Yeast

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKP3915

Plasmid

#113335

Purpose

Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Publication

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Artificial Transcription Factor (McIsaac et al, 2013)

Expression

Yeast

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pUG-natNT2

Plasmid

#110922

Purpose

expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

Depositor

Jean-Marc Daran

Publication

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

nat

Expression

Bacterial and Yeast

Promoter

AgTEF1

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pH3mCHSH2

Plasmid

#101053

Purpose

This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

Depositor

Jennifer Lodge

Publication

Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

Insert

Cryptococcus mCherry expression plasmid

Expression

Yeast

Promoter

Cryptococcus Histone3 promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YEp181-CUP1-His-Ubi

Plasmid

#99539

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

Depositor

Helle Ulrich

Publication

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pPMVAd3

Plasmid

#98298

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

Depositor

Claudia Vickers

Publication

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A

Expression

Yeast

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-kanMX6

Plasmid

#89067

Purpose

genomic GBP epitope C-terminal tagging in S. pombe

Depositor

Quanwen Jin

Publication

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

GBP

Expression

Yeast

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

Plasmid

#86424

Purpose

PCR tagging module with yeast codon optimized mTurquoise2

Depositor

Sue Jaspersen

Publication

Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

Insert

mTurquoise2

Expression

Yeast

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Plasmid

#58439

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

Depositor

Joerg Stelling

Publication

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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