-
Plasmid#40942DepositorInsertGFP-LacI**
UseTagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKK01
Plasmid#64744PurposeYeast expression plasmid: phaA, phaB, phaCDepositorInsertphaA, phaB, phaC
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIYC08
Plasmid#64743PurposeYeast expression plasmid: acsL641P, ALD6, ADH2, ERG10DepositorInsertADH2, ALD6, acsL641P, ERG10
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-HsFECH
Plasmid#174524PurposeExpresses human ferrochelatase (FECH) in yeastDepositorInsertFECH with yeast HEM15 promoter and terminator (FECH Human, Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationLacks original mitochondrial targeting sequence (…PromoterHEM15 (yeast, 257 bp)Available sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HaloTag-KanMX6
Plasmid#87029PurposeTag S. pombe genes in C terminal with HaloTagDepositorInsertHaloTag
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_AXP_hrGFP
Plasmid#84613PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZJOM143
Plasmid#133667PurposeIntegration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.DepositorInsertp1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIM23
Plasmid#74643PurposeCentromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoterMET25Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(URA)
Plasmid#49460Purposeoverespression of Flp step-arrest mutant in yeast, URA selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAS9i
Plasmid#141249PurposeGalactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterGAL1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOsTIR1w/oGFP
Plasmid#102883PurposeExpresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertOsTIR1
UseTags3x mycExpressionYeastMutationPromoterADH1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB8788
Plasmid#126922PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1DepositorInsertXdCrtI and HMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBKWH-p53
Plasmid#133900PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorInsertp53 (Trp53 Mouse)
UseTagsExpressionYeastMutationPromoterT7Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH732-2µ-RLuc/staCFLuc
Plasmid#40607DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)
Plasmid#173454PurposeContains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHLUM
Plasmid#40276DepositorUseTagsExpressionYeastMutationPromoterendogenous LEU2, endogenous MET15/17, and endogen…Available sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD-CRY2PHR
Plasmid#28244PurposeExpresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activationDepositorInsertcry2 (CRY2 Mustard Weed)
UseTagsGal4BDExpressionYeastMutationPromoterAvailable sinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Tom7
Plasmid#66571PurposeConstitutively express mitochondrial localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsTom7 and mCherryExpressionYeastMutationPhyB portion contains aa's 1-908PromoterpADH1Available sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSSa26
Plasmid#186784PurposeExpression of reverse transactivator (CgrtTA)DepositorInsertCgrtTA
UseTagsExpressionYeastMutationPromoterScPGK1pAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymNeongreen-T2A-mCherry
Plasmid#125700PurposeProduces equimolar expression of yeast codon optimized mNeongreen and mCherryDepositorInsertymNeongreen-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only