8,086 results

1. Gal4BD-CRY2(535)

   Plasmid

   #75369

   Purpose

   Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Gal4BD

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p426*-SaSgH

   Plasmid

   #136994

   Purpose

   Helper plasmid for SaCas9 gRNA cloning and expression

   Depositor

   Huimin Zhao

   Article

   Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pV1093:SpRY

   Plasmid

   #172188

   Purpose

   Genome editing of C. albicans

   Depositor

   Doug Bernstein

   Article

   Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ube2w

   Plasmid

   #31432

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (UBE2W Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pUNIV backbone

   Plasmid

   #24705

   Depositor

   Cynthia Czajkowski

   Article

   Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

   Type

   Empty backbone

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Bacterial, Mammalian, and Y…

   Mutation

   Promoter

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW1661

   Plasmid

   #112035

   Purpose

   OsTIR1 expression cassette marked with URA

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   TIR1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PRS415-Gal1pr-sctetR-FokI

   Plasmid

   #44756

   Depositor

   Narendra Maheshri

   Article

   Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

   Insert

   sctetR-FokI (fokI Synthetic)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1pr

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC5GGv1

   Plasmid

   #194645

   Purpose

   pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

   Plasmid

   #116884

   Purpose

   FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RLR)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG306GPD-ccdB

    Plasmid

    #14140

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPRyl_D17

    Plasmid

    #84611

    Purpose

    Introduces DSB at D17 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPL5071_TEF1*-Cre_URA3

    Plasmid

    #60930

    Purpose

    Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAW212

    Plasmid

    #172196

    Purpose

    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

    Depositor

    Chang Liu

    Article

    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

    Insert

    p10B2-AT110
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    10B2 (P1 specific promoter)

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDuRCC-K

    Plasmid

    #81193

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below., Please view…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMZ374

    Plasmid

    #59896

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG426GAL-SLEV_NS2B-GS-NS3

    Plasmid

    #203510

    Purpose

    Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    poly SLEV NS2B-GS-NS3 (POLY )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBT9-ubiquitin

    Plasmid

    #196999

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    ubiquitin (UBC Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104910

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDR FLIP43 GPS1

    Plasmid

    #101084

    Purpose

    GA biosensor expression plasmid

    Depositor

    Alexander Jones, Wolf Frommer

    Article

    Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

    Insert

    GPS1
    

    Use

    Tags

    6HIS and cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDR-GW AT1.03

    Plasmid

    #28003

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    AT1.03
    

    Use

    Tags

    mVenus and mseCFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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