8,432 results

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pKL05

Plasmid

#104429

Purpose

super Ecliptic pHluorin in yeast expression vector

Depositor

Joel Kralj

Publication

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

Super Ecliptic pHluorin

Expression

Yeast

Promoter

Tef1

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymNeongreen-T2A-mCherry

Plasmid

#125700

Purpose

Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen-T2A-mCherry

Expression

Yeast

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTurquoise2

Plasmid

#118453

Purpose

Overexpression of yeast codon-optimized mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAH243

Plasmid

#121147

Purpose

Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

Depositor

Katsunori Tanaka

Publication

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Inserts

humanized Streptococcus pyogenes Cas9
gRNA expression module

Use

CRISPR

Tags

Flag

Expression

Yeast

Promoter

S.pombe rrk1 and nmt41

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-pro-alphaf(I)-E2-Crimson

Plasmid

#117660

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Publication

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf(I)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPH1-mEGFP

Plasmid

#105270

Purpose

Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-mEGFP.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vph1 portion (VPH1 Budding Yeast)

Tags

mEGFP

Expression

Yeast

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHES941

Plasmid

#87942

Purpose

Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

Depositor

Hana El-Samad

Publication

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

GAL4 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human, Synthetic)

Expression

Yeast

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHCA/GAL4(1-93).ER.VP16

Plasmid

#108216

Purpose

Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain

Depositor

Didier Picard

Publication

Louvion et al Gene 1993 Sep 6;131(1):129-34.

Insert

Gal4 DBD-ERalpha HBD-VP16

Expression

Yeast

Mutation

ERalpha HBD has G400V mutation

Promoter

ADH1

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pROS10

Plasmid

#107924

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMEL16

Plasmid

#107922

Purpose

HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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POM43-mTFP1

Plasmid

#69853

Purpose

N-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Publication

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1 minus start and stop codon

Expression

Yeast

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pVTU-Dest

Plasmid

#105088

Purpose

Yeast expression vector for Gateway cloning and SUS bridge assays

Depositor

Christopher Grefen

Publication

Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

Type

Empty backbone

Expression

Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mYFP-hphMX6

Plasmid

#105155

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-hphMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ155

Plasmid

#98411

Purpose

Pombe Expression Vector - nmt1 promoter with Sp.ura4 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-mCherry2Bx6

Plasmid

#105273

Purpose

Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-mCherry2Bx6.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps8 portion (VPS8 Budding Yeast)

Tags

mCherry2Bx6

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHES836

Plasmid

#89195

Purpose

Expresses mKate2 under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Publication

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-mKate2

Expression

Yeast

Promoter

pZ

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUDE735

Plasmid

#103024

Purpose

expression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Publication

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDIS3

Plasmid

#92173

Purpose

Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.

Depositor

Judith Berman

Publication

Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

Insert

NAT1

Use

Genbank: u03440.1

Expression

Yeast

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDUP3

Plasmid

#92174

Purpose

Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

Depositor

Judith Berman

Publication

Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

Insert

NAT1

Use

Genbank: u03440.1

Expression

Yeast

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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