8,265 results

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pGI3EM18

Plasmid

#135484

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-tdTomato ADH1t

Tags

tdTomato

Expression

Bacterial and Yeast

Promoter

SpunH2Bpr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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EZ-L175

Plasmid

#173025

Purpose

Encodes OptoAMP1 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8644

Plasmid

#126919

Purpose

Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDR-GW FLIPglu-600μΔ13V

Plasmid

#28001

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-600μΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

F16A in mglB

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pHS3

Plasmid

#81038

Purpose

HO endonuclease expression

Depositor

John McCusker

Insert

NATMX - HO endonuclease

Expression

Yeast

Promoter

TEF

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only
pDM291-tetR-tup11Δ70

Plasmid

#41027

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetR-tup11Δ70

Expression

Yeast

Mutation

tup11 starts at aa70

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-PEX3

Plasmid

#51572

Purpose

Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

PEX3 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pICE ADE2

Plasmid

#122876

Purpose

ADE2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB2988

Plasmid

#63638

Purpose

pCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pXP218

Plasmid

#26831

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo

Plasmid

#115421

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAT222

Plasmid

#40942

Depositor

Angela Taddei

Article

Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

Insert

GFP-LacI**

Tags

GFP(S65T, V163A) and SV40 NLS

Expression

Yeast

Mutation

LacI**/LacI2G(4 mutations) (see comment below); l…

Promoter

His3 promoter fragment

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX-(3×FLAG)-TEV-ProtA

Plasmid

#52692

Purpose

For C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtA

Depositor

Michael Nick Boddy

Insert

(3×FLAG)-TEV-ProtA

Expression

Yeast

Promoter

n/a

Available Since

April 30, 2014

Availability

Academic Institutions and Nonprofits only

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pYTK-DN5

Plasmid

#180286

Purpose

NatR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - NatR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPA-mCherry-Kan

Plasmid

#44950

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-mCherry

Expression

Yeast

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-5FLAG-bleMX6

Plasmid

#19344

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

bleMX6

Expression

Yeast

Available Since

Sept. 25, 2008

Availability

Academic Institutions and Nonprofits only

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LifeAct-mScarletI pDRF1-GW

Plasmid

#168085

Purpose

Expresses Lifeact-mScarletI to visualize actin in yeast

Depositor

Bas Teusink

Insert

LifeAct-mScarletI

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pETconNK

Plasmid

#81169

Purpose

pETconNK Empty Backbone

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Type

Empty backbone

Tags

c-myc

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDZ276 (pURA MET28 PCP-2x-yeGFP)

Plasmid

#35194

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

PCP-2x-yeGFP

Expression

Yeast

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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