8,173 results
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Plasmid#145426PurposeYeast Expression plasmid for SARS-CoV-2 ORF7bDepositorInsertSARS-CoV-2 ORF7b (ORF7b Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
G30, Cdc20-mCherry-AID tagging
Plasmid#130270PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombinationDepositorInsertCdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
p41Neo MagicFluor
Plasmid#58564Purposep41Neo carrying both mating type markers buffered by the Cyc1 terminatorDepositorInsertSte2p-Citrine, Cyc1 term, Ste3p-mCherry
ExpressionYeastAvailable SinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJCHph
Plasmid#185863PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB6577
Plasmid#106129PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH646-2µ-RLuc
Plasmid#40600DepositorInsertRenilla Luciferase
ExpressionYeastPromoterADH1Available SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMZ377
Plasmid#74214PurposeCombined sgRNA/Cas9 vector with LEU2 markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0662
Plasmid#133477PurposeExpression in fission yeast from promoter pact1, integrated at lys3 locusDepositorInsertpLys3(BstZ17I)-p(act1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
HEL binding scFv D44.1
Plasmid#111718PurposeYeast Surface Display of D44.1 as a reference starting point for designDepositorInsertD44.1
Tagscmyc tagExpressionYeastAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046632
Plasmid#153929PurposeYeast expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationwtPromoterpTEF1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pILGFP4H
Plasmid#104503PurposeUsed to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSaGAL1 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4J
Plasmid#104505PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL1 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4N
Plasmid#104509PurposeUsed to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSbGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4R
Plasmid#104513PurposeUsed to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSuGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LD102
Plasmid#69161PurposeIntegration of GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg2-EGFP
TagsEGFPExpressionYeastAvailable SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Num1-PH
Plasmid#71374PurposeFor analysis of PH domain localization in yeastDepositorInsertNum1-PH
TagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa2563-2692Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPTK016-3-RFP_Intra
Plasmid#84975PurposeType 3, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable SinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046619
Plasmid#149218PurposeYeast Expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
ExpressionYeastMutationwtPromoterpGAL1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFTK078
Plasmid#171350PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTxlnA ANIA_03613 terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK077
Plasmid#171349PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTpenDE Pc21g21370 terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK076
Plasmid#171348PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtif35 Pc22g19890 terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK085
Plasmid#171357PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtRNA[Leu] P. rubens Pol-III terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK086
Plasmid#171358PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK053
Plasmid#171325PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertalcR ANIA_08978 transcriptional activator (no intron)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK090
Plasmid#171362PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsert3'flanking region - penicillin gene cluster P. rubens
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK089
Plasmid#171361PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsert5'flanking region - penicillin gene cluster P. rubens
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK083
Plasmid#171355PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTu6 hom., P. rubens Pol-III terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK082
Plasmid#171354PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTu3 hom., Tutp25, P. rubens Pol-III terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK081
Plasmid#171353PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTactA ANIA_06542 P. rubens terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK075
Plasmid#171347PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTamdS ANIA_08777 terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK074
Plasmid#171346PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertVPR -NLS AD (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK073
Plasmid#171345PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertRTT109 HAT AD (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK072
Plasmid#171344PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertp300core HAT AD (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK071
Plasmid#171343PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteGPF-NLS fluorescent reporter (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK070
Plasmid#171342PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteYFP-NLS fluorescent reporter (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK069
Plasmid#171341PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteGFP-NLS fluorescent reporter (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK068
Plasmid#171340PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertVP64 AD (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK067
Plasmid#171339PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertVP16 AD (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK066
Plasmid#171338PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertGal4 AD (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK065
Plasmid#171337PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertB42 AD (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK064
Plasmid#171336PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertQF AD from QA-1F (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK063
Plasmid#171335PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertdSpCas9(m4) (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK062
Plasmid#171334PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertdSpCas9(m2) (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK061
Plasmid#171333PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertSpCas9 (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK060
Plasmid#171332PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertGal4D BD (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK059
Plasmid#171331PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertLexA DBD (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK058
Plasmid#171330PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertQF DBD from QA-1F (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK057
Plasmid#171329PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertdSpCas9(m4)-VPR-NLS
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK054
Plasmid#171326PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertQS (QA-1S) codon optimized, quinic acid repressor
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only