7,942 results

1. pYAL_HLA-A2_MART1

   Plasmid

   #171177

   Purpose

   Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

   Depositor

   Michael Birnbaum

   Article

   Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

   Insert

   MART1_HLA-A2*01 single-chain trimer fusion
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLSB-NAT

   Plasmid

   #166698

   Purpose

   Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   adh15 promoter and tRNA promoter

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTA-HUH

   Plasmid

   #221152

   Purpose

   HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

   Depositor

   Sheng Yang

   Article

   Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

   Insert

   HisG-URA3-HisG
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. JME4599

   Plasmid

   #129660

   Purpose

   NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS418

   Plasmid

   #11256

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZS157 CRISPEY RT/Cas9

   Plasmid

   #114454

   Purpose

   Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

   Depositor

   Hunter Fraser

   Article

   Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

   Insert

   SpCas9, Ec86-RT
   

   Use

   CRISPR

   Tags

   SV40-NLS

   Expression

   Yeast

   Mutation

   S. cerevisiae codon optimized

   Promoter

   GAL1-GAL10

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIW601-KmCRISPR

   Plasmid

   #98907

   Purpose

   K. marxianus CRISPR Plasmid for sgRNA cloning

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p415 TEF roGFP2‐Tsa2ΔCR

   Plasmid

   #83238

   Purpose

   The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

   Insert

   roGFP2-Tsa2dCr
   

   Use

   Tags

   roGFP2 fusion with Tsa2dCr

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1

   Plasmid

   #58433

   Purpose

   Induces Citrine expression from a promoter containing 2 lexA boxes in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)2-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYES2-HTH-HHT2

    Plasmid

    #86482

    Purpose

    yeast expression of HHT2 with His10-TEV-HA tag

    Depositor

    Akash Gunjan

    Article

    Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

    Insert

    HHT2 (HHT2 Budding Yeast)
    

    Use

    Tags

    HIS10-TEV-HA (HTH)

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLM494

    Plasmid

    #100539

    Purpose

    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

    Inserts

    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1, TDH3, TIP1, and ZEO1

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lifeact-3xmNG::LEU2

    Plasmid

    #227594

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::LEU2
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSII42H-TDH3pr

    Plasmid

    #194533

    Purpose

    High copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSII42H-TDH3pr-mNeon

    Plasmid

    #194539

    Purpose

    High copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    hphMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNSU299_GAL2

    Plasmid

    #166088

    Purpose

    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    GAL2 (GAL2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCL28-decoder

    Plasmid

    #159450

    Purpose

    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    PlexAtet-LexA-ER-B112-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PlexAtet

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCL66-decoder

    Plasmid

    #159453

    Purpose

    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pfus1mut-tetR-nls-malE-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfus1mut

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDRF1-GW ymNeongreen-T2A-mCherry

    Plasmid

    #125700

    Purpose

    Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeongreen-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAR-Ec633

    Plasmid

    #130873

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L477V, L640Y, I777K, W814N

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBEVY-GU

    Plasmid

    #51229

    Purpose

    bi-directional expression vector for yeast, galactose inducible, URA3 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1,10

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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