8,418 results

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YL-CashGem-BAT1-URA3-LINEAR

Plasmid

#174837

Purpose

pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

Depositor

Zengyi Shao

Publication

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

GPDp

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pNH605-CCAAT-YFP

Plasmid

#172132

Purpose

Fluorescent reporter for HAP complex activity

Depositor

Johannes Herrmann

Publication

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xCCAAT-YFP

Use

Vector for genomic integration into the yeast gen…

Expression

Yeast

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_10_dCas9_VPR_HC_sgRNA

Plasmid

#163712

Purpose

Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Publication

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_4_dCas9_VPR_sgRNA

Plasmid

#163709

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Publication

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EZ-L560

Plasmid

#173028

Purpose

Encodes OptoAMP2 circuit

Depositor

Jose Avalos

Publication

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)

Plasmid

#173455

Purpose

Contains mScarlet-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pATP416-pluxO5-pphlO6-crtYBI

Plasmid

#165977

Purpose

Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA

Depositor

Jun Ishii

Publication

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Inserts

Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

C1281A, G1698A and C66A, C1359T

Promoter

Synthetic promoter (pluxO5), Synthetic promoter (…

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Dcp2 FL

Plasmid

#163574

Purpose

express wild type Dcp2 at endogenous level in S.cerevisiae

Depositor

Roy Parker

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

DCP2 promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG

Plasmid

#158127

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Publication

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-HIS-C4

Plasmid

#160340

Purpose

High-copy expression vector for K. marxianus, histidine selection

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-HPH

Plasmid

#160014

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-URA

Plasmid

#160009

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-G418

Plasmid

#160007

Purpose

Low-copy expression vector for K. marxianus, G418 selection

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJL-mGold

Plasmid

#157997

Purpose

Expression of mGold in yeast cells

Depositor

Francois St-Pierre

Publication

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold

Expression

Yeast

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

pTDH(GAP promoter)

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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nuc-yHS1

Plasmid

#159166

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Publication

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1

Tags

SV40 NLS

Expression

Yeast

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mito-yHS1

Plasmid

#159153

Purpose

Mitochondrial labile heme reporter

Depositor

Amit Reddi

Publication

Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

Insert

mito-yHS1

Tags

COX4 pre-sequence

Expression

Yeast

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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yHS1-M7A,H102A

Plasmid

#159152

Purpose

Cytosolic labile heme reporter

Depositor

Amit Reddi

Publication

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

yHS1-M7A,H102A

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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FRP935

Plasmid

#159302

Purpose

Yeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selection

Depositor

Joerg Stelling

Type

Empty backbone

Expression

Yeast

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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HsSPHK1

Plasmid

#118091

Purpose

Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

Depositor

Kevin Lynch

Publication

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Homo sapiens Sphingosine Kinase 1

Tags

FLAG

Expression

Yeast

Promoter

GAL1, 10

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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