8,057 results

1. YIplac211-KAR2-msGFP2-HDEL

   Plasmid

   #160468

   Purpose

   Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   KAR2-msGFP2-HDEL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBHM2329

   Plasmid

   #171686

   Purpose

   pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   PCnU6-sgRNA_scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   C. neoformans U6

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIplac128-pOst1-ESCargo

   Plasmid

   #115421

   Purpose

   Expresses a regulatable secretory cargo for yeast at the LEU2 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LifeAct-mScarletI pDRF1-GW

   Plasmid

   #168085

   Purpose

   Expresses Lifeact-mScarletI to visualize actin in yeast

   Depositor

   Bas Teusink

   Insert

   LifeAct-mScarletI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. RCP83

   Plasmid

   #163466

   Purpose

   Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

   Depositor

   Frank Albert

   Article

   Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

   Inserts

   Promoter fragment (Sharon2012_1922)
   His3 minimal promoter
   yeGFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG413GPD-ccdB

   Plasmid

   #14142

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG414GPD-ccdB

   Plasmid

   #14144

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-6xGLY-FLAG-kanMX6

   Plasmid

   #20751

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGR595

   Plasmid

   #213785

   Purpose

   Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

   Depositor

   Chang Liu

   Insert

   BadBoy2 TP-DNAP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ampl43

    Plasmid

    #161830

    Purpose

    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-6xGLY-V5-hphMX4

    Plasmid

    #20779

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-6xGLY-V5-kanMX6

    Plasmid

    #20780

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-6xGLY-Myc-hphMX4

    Plasmid

    #20770

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-6xGLY-FLAG-hphMX4

    Plasmid

    #20752

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR_XPR2_hrGFP

    Plasmid

    #84614

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPRyl_XPR2

    Plasmid

    #84609

    Purpose

    Introduces DSB at XPR2 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-link-ymScarletI3-Kan

    Plasmid

    #224077

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection

    Depositor

    Bas Teusink

    Insert

    ymScarletI3
    

    Use

    Tags

    ymScarletI3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p405ADH1

    Plasmid

    #15966

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    p405ADH1 (ADH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    ADH1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGS_101

    Plasmid

    #160541

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

    Depositor

    Li-Huei Tsai, Susan Lindquist

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Use

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    GAL1

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT040

    Plasmid

    #67640

    Purpose

    URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Insert

    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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