8,057 results

1. pAG425GPD-ccdB-HA

   Plasmid

   #14250

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHES840

   Plasmid

   #89191

   Purpose

   Expresses YFP under control of GAL1 promoter.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pGAL1-YFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LPH2782 - Ub-K48R

   Plasmid

   #32161

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K48R

   Promoter

   CUP1

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

   Plasmid

   #178985

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-mMaple3-kanMX6

   Plasmid

   #105165

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

   Insert

   mMaple3-kanMX6
   

   Use

   Tags

   GlySerGly-mMaple3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pI4-MTU-DO-G418

   Plasmid

   #160215

   Purpose

   Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). G418 selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIp ADE2-HphMx

   Plasmid

   #122916

   Purpose

   ADE2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pACT-Lex

   Plasmid

   #183149

   Purpose

   Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

   Depositor

   Assaf Mosquna

   Article

   Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

   Insert

   GAL4 AD
   

   Use

   Yeat two-hybrid

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAH243

   Plasmid

   #121147

   Purpose

   Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Inserts

   humanized Streptococcus pyogenes Cas9
   gRNA expression module
   

   Use

   CRISPR

   Tags

   Flag

   Expression

   Yeast

   Mutation

   Promoter

   S.pombe rrk1 and nmt41

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNB778

    Plasmid

    #60154

    Purpose

    Green click beetle luciferase (Promega) driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    CBG99
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    LEU1pr

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pESC-URA-Ub-G76A-Rnq1-GFP

    Plasmid

    #21052

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP and ubiquitin

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pJZC545

    Plasmid

    #62313

    Purpose

    sgRNA with 1x MS2 for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x MS2 binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGBW-m4046664

    Plasmid

    #149224

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4046660

    Plasmid

    #149214

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046689

    Plasmid

    #149212

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pHES941

    Plasmid

    #87942

    Purpose

    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    GAL4 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human, Synthetic)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAWH

    Plasmid

    #133894

    Purpose

    Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBKWH

    Plasmid

    #133898

    Purpose

    Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDB24

    Plasmid

    #91959

    Purpose

    empty expression vector for 10His-SUMO tag protein purification

    Depositor

    Christopher Bahl

    Type

    Empty backbone

    Use

    Tags

    10-His and Smt3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p426 25Q GPD

    Plasmid

    #1181

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only