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pDONR221_SLC16A14

Plasmid

#131926

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A14 (SLC16A14 Human)

Expression

Mammalian

Available since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221_SLC17A4

Plasmid

#131924

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC17A4 (SLC17A4 Human)

Expression

Mammalian

Available since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221_SLC17A3

Plasmid

#131912

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC17A3 (SLC17A3 Human)

Expression

Mammalian

Available since

Oct. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221_SLC13A1

Plasmid

#131909

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC13A1 (SLC13A1 Human)

Expression

Mammalian

Available since

Oct. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221_RHBG

Plasmid

#131883

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

RHBG (RHBG Human)

Expression

Mammalian

Available since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pSELt_S7.A

Plasmid

#125835

Purpose

Negative selection plasmid for evolution of an amber stop codon suppression tRNA

Depositor

Andrew Ellington

Publication

Maranhao et al ACS Synth Biol. 2017 Jan 20;6(1):108-119

Insert

ACP-2xAmb-pheS

Use

Synthetic Biology

Expression

Bacterial

Promoter

pr.trpL*

Available since

May 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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5W9S (CXXC5)

Plasmid

#101266

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CXXC5 (CXXC5 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available since

April 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1951

Plasmid

#105868

Purpose

High copy number plasmid containing Hspa1a (synonym Hsp68) MiniPromoter insert

Depositor

Elizabeth Simpson

Publication

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Hspa1a (synonym Hsp68) MiniPromoter (HSPA1A, HSPA1A Human)

Promoter

Hsp68 MiniPromoter

Available since

Feb. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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6D1T (MBD1)

Plasmid

#119966

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

MBD1 (MBD1 Human)

Tags

GSTtag, 6xHIS and Thrombin

Expression

Bacterial

Promoter

T7

Available since

Jan. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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6CCR (GID4)

Plasmid

#119960

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

GID4 (C17orf39 Human)

Tags

6xHIS, thrombin,SUMO and TEV cleavage

Expression

Bacterial

Mutation

contains amino acids 116-300

Promoter

T7

Available since

Jan. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK619

Plasmid

#110619

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-UBE3BΔIQ-HA-IRES-Puro

Plasmid

#107350

Purpose

Expresses HA tagged UBE3B deletion of IQ domain in mammalian cells.

Depositor

Robert Sobol

Publication

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3BdelIQ (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCT-CMV-UBE3BΔHECT-copGFP-Puro

Plasmid

#107343

Purpose

Expresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Publication

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(ΔHECT)-copGFP (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

deletion of HECT domain (757-end)

Promoter

CMV

Available since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pHS18 (mANGPTL3-Strep)

Plasmid

#109111

Purpose

Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

Depositor

Brandon Davies

Publication

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL3 (Angptl3 Mouse)

Tags

StrepII

Expression

Mammalian

Promoter

CMV

Available since

June 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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5KCH (SETDB1)

Plasmid

#101881

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SETDB1 (SETDB1 Human)

Tags

6xHis, TEV

Expression

Bacterial

Promoter

T7

Available since

April 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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AGPAT3_pCSdest

Plasmid

#53928

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

AGPAT3 (AGPAT3 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
Col18a1_pCSdest

Plasmid

#53930

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

Col18a1 (Col18a1 Mouse)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
TRAPPC10_pCSdest

Plasmid

#53937

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TRAPPC10 (TRAPPC10 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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ERG_pCSdest

Plasmid

#53864

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ERG (ERG Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
HSPA13_pCSdest

Plasmid

#53872

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

HSPA13 (HSPA13 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits