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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 505 results for: SPY

Showing: 1 - 10 of 505 results

  1. SpyTag-β-Lactamase-SpyCatcher

    Plasmid
    #52656
    Purpose
    Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.
    Depositor
    Mark Howarth
    Article
    Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2
    Insert
    TEM-1 β-Lactamase
    Use
    Tags
    SpyCatcher and SpyTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52656-plasmid-map-sequence-id-422741

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  3. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid
    #216283
    Purpose
    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SpyCatcher003-TEVs-SpyTag003DA
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216283-plasmid-map-sequence-id-433206

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  7. pExp-Spy

    Plasmid
    #129240
    Purpose
    Protein expression in E.coli
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    His and Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    129240-plasmid-map-sequence-id-252870

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Showing: 1 - 10 of 505 results