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Showing: 1 - 40 of 360 results

  1. pAf-CRISPR-yA

    Plasmid
    #191015
    Purpose
    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.
    Depositor
    Perng-Kuang Chang
    Article
    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046
    Insert
    yA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Aspergillus flavus U6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    191015-plasmid-map-sequence-id-382132

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  3. pdcexADN

    Plasmid
    #227344
    Purpose
    Deletion of the AocexA gene in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Flanking regions of AocexA (AO090009000645)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227344-plasmid-map-sequence-id-458569

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  4. pPtef1-aclB

    Plasmid
    #227343
    Purpose
    Overexpression of the aclB gene in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Expression cassettes of AoaclB (AO090023000206)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227343-plasmid-map-sequence-id-458566

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  5. pPtef1-aclA

    Plasmid
    #227342
    Purpose
    Overexpression of the aclA gene in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Expression cassettes of AoaclA (AO090023000205)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227342-plasmid-map-sequence-id-458619

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  6. pwAB-erg10erg13

    Plasmid
    #227346
    Purpose
    Overexpression of the erg10 and erg13 genes in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Expression cassettes of the erg10 and erg13 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227346-plasmid-map-sequence-id-458526

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  7. pTOCK1

    Plasmid
    #215566
    Purpose
    Escherichia coli–Aspergillus spp. shuttle cosmid-based library system
    Depositor
    Takuji Oka
    Article
    Kadooka et al J Fungi (Basel). 2024 Feb 29;10(3):188.
    Inserts
    AopyrG
    AMA1
    ampicillin resistance (bla) ORF
    Use
    Escherichia coli-aspergillus spp. shuttle cosmid …
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    215566-plasmid-map-sequence-id-428769

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  8. pwAC-trAO217erg12

    Plasmid
    #227347
    Purpose
    Overexpression of the truncated AO217 and erg12 genes in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Expression cassettes of the truncated AO217 and erg12 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227347-plasmid-map-sequence-id-458527

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  9. pwAB-erg8erg19idi1erg20

    Plasmid
    #227345
    Purpose
    Overexpression of the erg8, erg19, idi1, and erg20 genes in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Expression cassettes of the erg8, erg19, idi1, and erg20 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227345-plasmid-map-sequence-id-458568

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  10. pXylP-HygR

    Plasmid
    #168131
    Purpose
    Expression of HygR from the xylose-inducible xylP promoter
    Depositor
    Michael Bromley
    Insert
    XylP-HygR
    Use
    Aspergillus expression
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    xylP
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    168131-plasmid-map-sequence-id-329723

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  11. pHS201-ophi

    Plasmid
    #227349
    Purpose
    Heterologous production of ophiobolin C in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    The hoomlogous regions of the HS201 locus and expression cassettes of the AcOS, oblDBm, and oblBBm genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227349-plasmid-map-sequence-id-459986

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  12. pdadhDN-tefAO217

    Plasmid
    #227341
    Purpose
    Replacement of the adh gene with the expression cassette of the AO217 gene
    Depositor
    Jun-ichi Maruyama
    Insert
    Flanking regions of adh (AO090009000634) and expression cassette of AO217 (AO090120000217)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227341-plasmid-map-sequence-id-458620

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  22. ppG-ple12

    Plasmid
    #227339
    Purpose
    Heterologous production of pleuromutilin in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    The upstream/downstream regions of the pyrG gene and expression cassettes of the ple1 and ple2 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    227339-plasmid-map-sequence-id-458622

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  23. pwA-ple34

    Plasmid
    #227337
    Purpose
    Heterologous production of pleuromutilin in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    The upstream/inside regions of the wA gene and expression cassettes of the ple3 and ple4 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    227337-plasmid-map-sequence-id-458564

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  24. pDIV303

    Plasmid
    #204978
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    amdS
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    two silent mutations (nt177T>C, nt879C>T)
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204978-plasmid-map-sequence-id-415579

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  25. pSF8

    Plasmid
    #222592
    Purpose
    BB2 gsdA tet-on overexpression cassette
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    gsdA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert,…
    Promoter
    tet-on
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222592-plasmid-map-sequence-id-443808

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  26. pGem-LP1-loxP-bar-lox2272-71

    Plasmid
    #168785
    Purpose
    Vector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Inserts
    bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    loxP
    lox2272-71
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168785-plasmid-map-sequence-id-350000

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  27. pGem-LP2-loxP-bar-lox2272-71

    Plasmid
    #168786
    Purpose
    Vector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Inserts
    bar
    IS1 Homology arm 1
    IS1 Homology arm 2
    loxP
    lox2272-71
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168786-plasmid-map-sequence-id-353782

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  28. pFC330

    Plasmid
    #87842
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    pyrG
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    87842-plasmid-map-sequence-id-189665

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  29. pDM067

    Plasmid
    #216820
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold2
    Use
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216820-plasmid-map-sequence-id-430143

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  30. pFC331

    Plasmid
    #87843
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87843-plasmid-map-sequence-id-177947

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  31. pDM042

    Plasmid
    #216812
    Purpose
    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mScarlet3
    Use
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216812-plasmid-map-sequence-id-429538

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  32. pDM043

    Plasmid
    #216813
    Purpose
    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mScarlet3
    Use
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216813-plasmid-map-sequence-id-430146

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  33. pDM052

    Plasmid
    #216814
    Purpose
    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mNeonGreen
    Use
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216814-plasmid-map-sequence-id-429539

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  34. pDM053

    Plasmid
    #216815
    Purpose
    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mNeonGreen
    Use
    Tags
    RIPGLIN
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216815-plasmid-map-sequence-id-429382

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  35. pDM070

    Plasmid
    #216816
    Purpose
    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mCardinal
    Use
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216816-plasmid-map-sequence-id-429610

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  36. pDM071

    Plasmid
    #216817
    Purpose
    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mCardinal
    Use
    Tags
    RIPGLIN
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216817-plasmid-map-sequence-id-430145

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  37. pDM069

    Plasmid
    #216821
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold2
    Use
    Tags
    RIPGLIN and VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216821-plasmid-map-sequence-id-430130

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  38. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

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  39. pDIV298

    Plasmid
    #204957
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    pyrG
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204957-plasmid-map-sequence-id-415603

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  40. pDM098

    Plasmid
    #216818
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold
    Use
    Tags
    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216818-plasmid-map-sequence-id-429609

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Showing: 1 - 40 of 360 results