Skip to main content
Addgene
Showing: 1 - 50 of 93 results

  26. pSRF-donor

    Plasmid
    #112362
    Purpose
    CRISPR donor plasmid to tag human transcription factor SRF with GFP
    Depositor
    Kevin White
    Insert
    SRF homology arms flanking EGFP-IRES-Neo cassette (SRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:6:43178470:C>A, hg38:6:43178514:G>A
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112362-plasmid-map-sequence-id-229685

    Enlarge

  28. FLAG-hSRF (#482)

    Plasmid
    #78343
    Purpose
    Overexpression of human SRF ORF with a N-terminal FLAG tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. pQUASp-RedStinger (nlsRFP)

    Plasmid
    #46165
    Purpose
    Expression of RedStinger in insect
    Depositor
    Christopher Potter
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    46165-plasmid-map-sequence-id-65049

    Enlarge

  30. RSRF-Luc-2mut

    Plasmid
    #31819
    Depositor
    Astar Winoto
    Article
    Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.
    Insert
    Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    two CTATATTTAG RSRF sites mutated to CGATATTTCG
    Promoter
    mutated MEF2-dependent (-307 to -242 of Nur77/NR4…
    Available since
    Aug. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  34. pSRF.1.0-gDNA

    Plasmid
    #112458
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SRF
    Depositor
    Kevin White
    Insert
    SRF (SRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112458-plasmid-map-sequence-id-230474

    Enlarge

  35. pLenti.CAG.H2B-cerFP-2A-mito-dsRFP.W

    Plasmid
    #51013
    Purpose
    Lentivector that expresses H2B-cerFP-2A-mito-dsRFP from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-cerFP-2A-mito-RFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51013-plasmid-map-sequence-id-77778

    Enlarge

  36. pINDUCER20-FLAG-hSRF (#799)

    Plasmid
    #78345
    Purpose
    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a FLAG tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  37. pINDUCER20-hSRF-3xHA (#757)

    Plasmid
    #78344
    Purpose
    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a 3xHA tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  39. hSRF-3xHA-IRES-hrGFP (#479)

    Plasmid
    #78347
    Purpose
    Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  40. KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

    Plasmid
    #121835
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121835-plasmid-map-sequence-id-237389

    Enlarge

  41. KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300core

    Plasmid
    #121839
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121839-plasmid-map-sequence-id-237393

    Enlarge

  42. KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR

    Plasmid
    #121836
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121836-plasmid-map-sequence-id-237390

    Enlarge

  43. KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

    Plasmid
    #121837
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121837-plasmid-map-sequence-id-237391

    Enlarge

  44. KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1

    Plasmid
    #121838
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121838-plasmid-map-sequence-id-237392

    Enlarge

  45. KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

    Plasmid
    #121840
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121840-plasmid-map-sequence-id-237394

    Enlarge

Showing: 1 - 50 of 93 results