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Plasmid#60733PurposepET28 driving expression of GFP, biobrick part Bba_E0040DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-iLOVf
Plasmid#63723PurposeE coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteinsDepositorTypeEmpty backboneUseTagsTEE, iLOV, 6xHis, thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL
Plasmid#26096PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-SaCas9
Plasmid#178901PurposeSaCas9 (Cas9 from Staphylococcus aureus)DepositorInsertSaCas9
UseTagsHis6-Tag, bipartite nuclear localization signal f…ExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET28-2H12
Plasmid#221186PurposeHis6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)DepositorInsertcdGreen
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV
Plasmid#208635Purposefor E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.DepositorTypeEmpty backboneUseTags6xhisExpressionBacterialMutationPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-hTDG
Plasmid#70758Purposebacterial expression of human TDGDepositorInsertTDG (TDG Human)
UseTags6-HISExpressionBacterialMutationPromoterT7/lac operonAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-hXRCC1
Plasmid#70759Purposebacterial expression of human XRCC1DepositorInsertXRCC1 (XRCC1 Human)
UseTags6-HISExpressionBacterialMutationPromoterT7/lac operonAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKH8SUMO
Plasmid#79526PurposeBacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.DepositorTypeEmpty backboneUseTags8xHis-Thrombin-SUMO-TEV and TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 16, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-Rsn2
Plasmid#61686Purposebacterial expression of ranaspumin 2 as a His-tagged fusion proteinDepositorInsertRanaspumin-2
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-OmpT
Plasmid#68862Purposeconstruct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.DepositorInsertOmpT (ompT )
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP1
Plasmid#67862Purposebacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)DepositorInsertMRPP1 (TRMT10C Human)
UseTagsHisExpressionBacterialMutationmitoch. form (contains aa 40-403)PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-HADH2
Plasmid#67863Purposebacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)DepositorInsertHADH2 (HSD17B10 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-Kss1
Plasmid#52682PurposeExpression of Kss1 in bacteriaDepositorInsertKSS1 (KSS1 Budding Yeast)
UseTagsHis6 and T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28(+)-NudC
Plasmid#175022PurposePurification of NudC enzymeDepositorInsertnudC
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-mHP1alpha
Plasmid#181911PurposeBacterial protein expression vector for HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFLtail
Plasmid#232055PurposeExpresses construct for surface tethering: R. norvegicus NFL tail domainDepositorInsertNeurofilament-Light tail domain
UseTags6xHisExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
UseTags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf1
Plasmid#232058PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1DepositorInsertNeurofilament-Medium tail domain, charge shuffle 1
UseTags6xHis, thrombinExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHtail
Plasmid#232057PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domainDepositorInsertNeurofilament-Heavy tail domain
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf2
Plasmid#232059PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2DepositorInsertNeurofilament-Medium tail domain, charge shuffle 2
UseTags6xHis, thrombinExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII
Plasmid#225075PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII
Plasmid#225066PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Butelase
Plasmid#221848PurposeExpresses asparaginyl endopeptidase butelaseDepositorInsertButelase 1 ligase
UseTagsExpressionBacterialMutationNoPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-2H12Matry
Plasmid#221187PurposeHis6-tagged cdGreen-Matry expressed from T7 promoter (Internal Lab ID: UJ11309)DepositorInsertcdGreen-Matry
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MetN
Plasmid#114458PurposeExpression of hexahistidine-tagged AceS methylase in E. coliDepositorInsertaceS
UseTagsHexahistidineExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-XI
Plasmid#157988PurposeExpresses XI in E.coli cellDepositorInsertxylose isomerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28aAviHisCD72cCTLD
Plasmid#129066Purposegeneration of biotinylated mouse CD72c CTLD protein using bacteriaDepositorInsertCD72c (Cd72 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
ERG_pet28a
Plasmid#131642PurposeBacterial expression of ETS gene ERGDepositorInsertERG (ERG Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
NylCK_pET28a
Plasmid#215422PurposeExpression construct for NylCK gene, codon optimized for expression in E. coliDepositorInsertNylCK
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCA_pET28a
Plasmid#215418PurposeExpression construct for NylCA gene, codon optimized for expression in E. coliDepositorInsertNylCA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2_pET28a
Plasmid#215420PurposeExpression construct for NylCp2 gene, codon optimized for expression in E. coliDepositorInsertNylCp2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_hTLN2-FL
Plasmid#164835PurposeExpression of FL human talin2DepositorInsertTLN2 (TLN2 Human)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
ETS1_pet28a
Plasmid#131663PurposeBacterial expression of ETS gene ETS1DepositorInsertETS1 (ETS1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
ETV4_pet28a
Plasmid#131645PurposeBacterial expression of ETS gene ETV4DepositorInsertETV4 (ETV4 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
ETV5_pet28a
Plasmid#131639PurposeBacterial expression of ETS gene ETV5DepositorInsertETV5 (ETV5 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLI1_pet28a
Plasmid#131641PurposeBacterial expression of ETS gene FLI1DepositorInsertFLI1 (FLI1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
ELK1_pet28a
Plasmid#131647PurposeBacterial expression of ETS gene ELK1DepositorInsertELK1 (ELK1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY1
Plasmid#21760DepositorInsertECFP -EYFP linked by flexible one SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H4
Plasmid#42633DepositorInsertHuman H4 (H4C1 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pet28a_Synthetic_Human_H2A.1
Plasmid#42634DepositorInsertHuman H2A.1 (H2AC13 Synthetic, Human)
UseTagsUntaggedExpressionBacterialMutationCodon OptimizedPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a_PTK2b
Plasmid#224237PurposeExpression of human PTK2b Kinase in E. coliDepositorInsertptk2b (PTK2B Human)
UseTags6xHis and 6xHis-TEVExpressionBacterialMutationPromoterT7 promotorAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H3.1
Plasmid#42631DepositorInsertHuman H3.1 (H3C1 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a_Pro-IL-1β
Plasmid#214340PurposeProtein overexpression in bacteriaDepositorInsertIL1B (IL1B Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_SYK
Plasmid#224240PurposeExpression of human SYK Kinase in E. coliDepositorInsertsyk (SYK Human)
UseTags6xHis and 6xHis-TEVExpressionBacterialMutationPromoterT7 promotorAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LYN
Plasmid#224238PurposeExpression of human LYN Kinase in E. coliDepositorInsertlyn (LYN Human)
UseTags6xHis and 6xHis-TEVExpressionBacterialMutationPromoterT7 promotorAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H2B.1
Plasmid#42630DepositorInsertHuman H2B.1 (H2BC8 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a_TIA1
Plasmid#106095Purposebacterial protein expression of TIA1DepositorInsertTIA1 (TIA1 Human)
UseTags6xHIs-thrombin and T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_NoMTS_pET28
Plasmid#34705DepositorInsertTFAM (TFAM Human)
UseTags6XHisExpressionBacterialMutationEncodes residues 43–246, corresponding to full-le…PromoterT7Available sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only