We narrowed to 54 results for: tol2 transposon

1. pKTol2C-EGFP

   Plasmid

   #85598

   Purpose

   Tol2 transposon with EGFP driven by minimal-CAGs promoter.

   Depositor

   Scott Fahrenkrug, Karl Clark

   Article

   Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

   Insert

   Tol2 transposon with EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mini-CAG

   Available since

   Dec. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKTol2P-PTK

   Plasmid

   #85599

   Purpose

   Tol2 transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

   Depositor

   Scott Fahrenkrug, Karl Clark

   Article

   Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

   Insert

   Tol2 transposon with a puromycin-thymidine kinase fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Sept. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKC-Tol2

   Plasmid

   #85600

   Purpose

   Source of Tol2 transposase driven by a mini-CAGS promoter.

   Depositor

   Scott Fahrenkrug, Karl Clark

   Article

   Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

   Insert

   Tol2 transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mini-CAG

   Available since

   Dec. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKUb-Tol2

   Plasmid

   #85601

   Purpose

   Source of Tol2 transposase driven by a human ubiquitin promoter.

   Depositor

   Scott Fahrenkrug, Karl Clark

   Article

   Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

   Insert

   Tol2 transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Ubiquitin

   Available since

   Feb. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-Tol2

   Plasmid

   #31823

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT3TS-Tol2

   Plasmid

   #31831

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Lac

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pminiTol2

   Plasmid

   #31829

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAK_Tol2_TRE_Puro

   Plasmid

   #130259

   Purpose

   Tol2 doxycycline inducible expression vector

   Depositor

   Michael Yaffe

   Type

   Empty backbone

   Use

   Tol2 transposon, doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAK_Tol2_TRE_Hygro

   Plasmid

   #130260

   Purpose

   Tol2 doxycycline inducible expression vector

   Depositor

   Michael Yaffe

   Type

   Empty backbone

   Use

   Tol2 transposon, doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE Promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAK_Tol2_TRE_Blast

    Plasmid

    #130261

    Purpose

    Tol2 doxycycline inducible expression vector

    Depositor

    Michael Yaffe

    Type

    Empty backbone

    Use

    Tol2 transposon, doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAK_Tol2_TRE_Zeo

    Plasmid

    #130263

    Purpose

    Tol2 doxycycline inducible expression vector

    Depositor

    Michael Yaffe

    Type

    Empty backbone

    Use

    Tol2 transposon, doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAK_Tol2_TRE_Neo

    Plasmid

    #130262

    Purpose

    Tol2 doxycycline inducible expression vector

    Depositor

    Michael Yaffe

    Type

    Empty backbone

    Use

    Tol2 transposon, doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Tol2-U6.3-sgRNA-DNM1L -GFP

    Plasmid

    #221845

    Purpose

    Tol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGC and U6.3 chick

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Tol2-U6.3-sgRNA-MFN1-GFP

    Plasmid

    #221846

    Purpose

    Tol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promote

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGC and U6.3 chick

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid

    #221844

    Purpose

    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GACG and U6.3 chick

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDestTol2pA2_ubi:EGFP

    Plasmid

    #27323

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    ubiquitin:EGFP reporter
    

    Use

    Tol2 transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDestTol2-ubi-ASAP1

    Plasmid

    #121987

    Purpose

    ubiqutously express ASAP1 voltage biosensor

    Depositor

    GuangJun Zhang

    Article

    Silic et al J Vis Exp. 2018 Apr 25;(134). doi: 10.37

    Insert

    ASAP1
    

    Use

    Tol2 transposon destination vector

    Tags

    Expression

    Mutation

    Promoter

    zebrafish ubiquitin promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Tol2-U6.3-sgRNA-empty-GFP

    Plasmid

    #221843

    Purpose

    empty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Insert

    EGFP
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGC

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTol2-CreLite

    Plasmid

    #131783

    Purpose

    Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC, PIF6CreN, and mTagBFP2
    

    Use

    Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Ubi

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTol2CG NBT:NRGIIIa:polyA

    Plasmid

    #193012

    Purpose

    Tol2 transgenic construct that will express human Neuregulin1 Type IIIa in neurons

    Depositor

    William Talbot

    Article

    Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

    Inserts

    NBT
    NRG1 type IIIa (NRG1 Human)
    

    Use

    Transposon transgenesis

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTol2CG NBT:NRGIIIa_R551Q:polyA

    Plasmid

    #193013

    Purpose

    Tol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neurons

    Depositor

    William Talbot

    Article

    Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

    Inserts

    NBT
    NRG1 type IIIa R551Q (NRG1 Human)
    

    Use

    Transposon transgenesis

    Tags

    Expression

    Mammalian

    Mutation

    ENST00000287842, NM_013956, c.1652G>A, p.(Arg5…

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Tol2-elavl3-GCaMP6s

    Plasmid

    #59531

    Purpose

    Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    GCaMP6s
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Tol2-CAG::Cytbow

    Plasmid

    #158991

    Purpose

    Multicolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Cytbow
    

    Use

    Tags

    The default expressed EBFP2 is fused to H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Tol2-CAG::Nucbow

    Plasmid

    #158992

    Purpose

    Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Nucbow
    

    Use

    Tags

    All FPs are fused to H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Tol2-CAG::Palmbow

    Plasmid

    #158993

    Purpose

    Multicolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Palmbow
    

    Use

    Tags

    The default expressed EBFP2 is fused to H2B; oth…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Tol2-CAG::Mitbow

    Plasmid

    #158994

    Purpose

    Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Mitbow
    

    Use

    Tags

    The default expressed EBFP2 is fused to H2B; oth…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Tol2-elavl3-H2B-GCaMP6s

    Plasmid

    #59530

    Purpose

    Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    H2B
    GCaMP6s
    

    Use

    Transposable element

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDestTol2-actinb-kcnj13-EGFP

    Plasmid

    #164940

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted

    Promoter

    actinb (actb2) promoter

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTol2-PcyA-IRES-HO1

    Plasmid

    #131787

    Purpose

    Tol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PcyA and HO1
    

    Use

    Synthetic Biology; Zebrafish tol2 transposon

    Tags

    MTS (Mitochondrial Targeting Signal from subunit …

    Expression

    Mammalian

    Mutation

    Promoter

    hsp70

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDestTol2-actinb-kcna1a-IRES-EGFP

    Plasmid

    #164965

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcna1a (kcna1a Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDestTol2-actinb-kcnj10a-IRES-EGFP

    Plasmid

    #164947

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj10a (kcnj10a Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDestTol2-actinb-kcnk9-IRES-EGFP

    Plasmid

    #164949

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnk9 (kcnk9 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDestTol2-pax3a-kcnj13-IRES-EGFP

    Plasmid

    #164950

    Purpose

    Tol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    pax3a promoter -5.4k

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDestTol2-actinb-kcnj13-IRES-EGFP

    Plasmid

    #164941

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDestTol2-actinb-kcnj13-M135R-EGFP

    Plasmid

    #164944

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish gain-of-function kcnj13 mutant, M135R

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted; M135R

    Promoter

    actinb (actb2) promoter

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

    Plasmid

    #164945

    Purpose

    Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    KCNJ13 (KCNJ13 Human)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Stop codon deleted

    Promoter

    actinb (actb2) promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDestTol2-actinb-kcnj13-Q153H-EGFP

    Plasmid

    #164943

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted; Q153H; M154I

    Promoter

    actinb (actb2) promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDestTol2-actinb-kcnj13-T131A-EGFP

    Plasmid

    #164942

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted; T131A; G133R

    Promoter

    actinb (actb2) promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDestTol2-actinb-kcnj1b-IRES-EGFP

    Plasmid

    #164946

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj1b (kcnj1b Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

    Plasmid

    #168108

    Purpose

    Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pT2MP.EF-QUE37C

    Plasmid

    #129332

    Purpose

    ATP measurement, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pT2MP.EF-pHlu

    Plasmid

    #129304

    Purpose

    pH measurement, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    pHluorin (from H. Imamura)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pT2MPEF-TOMM-QUE37C

    Plasmid

    #191526

    Purpose

    ATP measurement, mitochondria outer membrane, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pT2MPEF-NLS-QUE37C

    Plasmid

    #129326

    Purpose

    ATP measurement, nucleus, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

46. pGBT-R16

    Plasmid

    #31827

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    mini Tol2 ITR
    

    Use

    Cre/Lox; Gene trap

    Tags

    mRFP

    Expression

    Mutation

    mRFP lacks start codon

    Promoter

    beta Actin

    Available since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

    Plasmid

    #71485

    Purpose

    Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

    Depositor

    Richard Sherwood

    Article

    Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pT2P-EF-Q7mu

    Plasmid

    #129303

    Purpose

    Positive control for ATP measurement, mitochondria outer membrane, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-7mu
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pT2MP.EF-QNA

    Plasmid

    #129306

    Purpose

    Negative control for ATP measurement, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-NA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pT2MP.EF-Q2m

    Plasmid

    #129310

    Purpose

    QUEEN ATP sensor for 25degC, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-2m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge