Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 2,838 results for: 52963

Clear keywords
Showing: 1 - 20 of 2838 results

  1. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52963-plasmid-map-sequence-id-331247

    Enlarge

These results may also be relevant.

  1. pGBW-m4252963

    Plasmid
    #153672
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp11
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153672-plasmid-map-sequence-id-300643

    Enlarge

  2. lentiGuide-Hygro-eGFP

    Plasmid
    #99375
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99375-plasmid-map-sequence-id-191025

    Enlarge

  4. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Publication
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    137729-plasmid-map-sequence-id-272646

    Enlarge

  5. lentiGuide-Hygro-dTomato

    Plasmid
    #99376
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99376-plasmid-map-sequence-id-191027

    Enlarge

  6. lentiGuide-Hygro-mTagBFP2

    Plasmid
    #99374
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99374-plasmid-map-sequence-id-430620

    Enlarge

  11. pCMV5-AML1B

    Plasmid
    #12426
    Purpose
    pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.
    Depositor
    Scott Hiebert
    Publication
    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AML1B (RUNX1 Human)
    Expression
    Mammalian
    Available since
    Nov. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12426-plasmid-map-sequence-id-151854

    Enlarge

  13. LentiGuide_DKO

    Plasmid
    #183193
    Purpose
    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Publication
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183193-plasmid-map-sequence-id-360099

    Enlarge

  16. lentiGuide-Hygro-iRFP670

    Plasmid
    #99377
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99377-plasmid-map-sequence-id-191028

    Enlarge

Showing: 1 - 20 of 2838 results