We narrowed to 215 results for: stem loop

1. pBS-Histone H3 imperfect loop

   Plasmid

   #45087

   Depositor

   Phillip Newmark

   Article

   Rouhana et al Development. 2014 Jul;141(13):2592-601.

   Insert

   Histone H3 imperfect stem
   

   Use

   RNAi; In vitro transcription

   Tags

   Expression

   Mutation

   stem loop sequence is naturally occurring mutant …

   Promoter

   T7 sense, T3 antisense

   Available since

   May 30, 2013

   Availability

   Academic Institutions and Nonprofits only

2. OpenCRISPR-1 sgRNA-16nt stem

   Plasmid

   #221566

   Purpose

   Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1 sgRNA 16nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. OpenCRISPR-1 sgRNA-12nt stem

   Plasmid

   #221567

   Purpose

   Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1 sgRNA 12nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pCDH-V1AUC-DelStem-CD20-Puro

   Plasmid

   #209758

   Purpose

   Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Mutations to disrupt the uORFs and the stem-loop …

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LSM075 SFFV-mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP (FLP-IN)

   Plasmid

   #233439

   Purpose

   SFFV driven LIDAR reporter with 2 tandem substrate stem loops

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LSM081 SFFV-mCherry-SGc(stem-1(3xMS2)2-stops)cSG-EGFP (FLP-IN)

   Plasmid

   #233440

   Purpose

   SFFV driven LIDAR reporter with an additional stop codon on the stem loop

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   SGc(stem-1(3xMS2)2-stops)cSG-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmax pona 12xTRICK 24xMS2SL

   Plasmid

   #64542

   Purpose

   TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   12x PP7 stem-loops
   

   Use

   Tags

   24xMS2 stem loops, HA, NLS, and dNP799

   Expression

   Mammalian

   Mutation

   Promoter

   ponA

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-Ef1a-RPS27A-UTR-PP7-WPRE

   Plasmid

   #198339

   Purpose

   PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   RPS27A-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-Ef1a-SunTag-RPS7-PP7-WPRE

   Plasmid

   #198338

   Purpose

   PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   GCN4x24-RPS7-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops and SunTag

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Pol

    Plasmid

    #65520

    Purpose

    Expresses HBV polymerase in mammalian cells

    Depositor

    Wang-Shick Ryu

    Article

    Ryu et al Virology. 2008 Mar 30;373(1):112-23. Epu

    Insert

    HBV polymerase protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 4xMS2-hTR

    Plasmid

    #154940

    Purpose

    4xMS2 stem-loop-hTR-500 bp genomic sequence

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    4xMS2 stem-loop-hTR-500 bp genomic sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. plIVMD156

    Plasmid

    #160934

    Purpose

    In vivo mRNA display Gateway Destination construct

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
    

    Use

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. plIVMD164

    Plasmid

    #160936

    Purpose

    In vivo mRNA display construct for GFP

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
    

    Use

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. plIVMD163

    Plasmid

    #160935

    Purpose

    In vivo mRNA display construct for mCherry

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
    

    Use

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6_(GLuc)_INT[3xMS2_SL]

    Plasmid

    #68426

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three MS2 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV/MASC_(GLuc)_INT

    Plasmid

    #68440

    Purpose

    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three PP7 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL2084

    Plasmid

    #137726

    Purpose

    Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1

    Depositor

    Jeremy Mottram

    Article

    Jones et al PLoS Pathog. 2014 Jan;10(1):e1003886. do

    Type

    Empty backbone

    Use

    RNAi; Trypanosoma brucei tet inducible stem loop

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJWK_VD_01

    Plasmid

    #158102

    Purpose

    5'UTR stem-loop luciferase reporter: 0kcal/mol

    Depositor

    Ted Dawson

    Article

    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

    Insert

    5'UTR synthetic stem-loop 0kcal/mol
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJWK_VD_02

    Plasmid

    #158103

    Purpose

    5'UTR stem-loop luciferase reporter: -30kcal/mol

    Depositor

    Ted Dawson

    Article

    Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

    Insert

    5'UTR synthetic stem-loop -30kcal/mol
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6_(GLuc)_INT[3xPP7_SL]

    Plasmid

    #68424

    Purpose

    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct bearing three PP7 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge