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Plasmid#182343PurposeEmpty cloning vector for mosTI(unc-119). MCS or Golden Gate (BsaI: tgcc - MCS - gagc)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only
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UbC-blasticidine-P2A-TETon-3G transactivator
Plasmid#200191PurposeA TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertblasticidine - P2A - TETon-3G transactivator
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterUbCAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-hPGK-Blast-2A-STING-TurboID
Plasmid#204713PurposeExpresses C-terminal TurboID tagged human STING for proximity ligation.DepositorInsertSTING1 (STING1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationPromoterhPGKAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-UbC-Blast-2A-STING-mNeonGreen
Plasmid#227185PurposeLentiviral expression plasmid encoding STING-mNeonGreen under UbC promoterDepositorInsertSTING1 (STING1 Human)
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterUbCAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMK444 (EF1-OsTIR1(F74G) Puro)
Plasmid#214374PurposeEF1-OsTIR1(F74G) (PiggyBac)DepositorInsertEF1-OsTIR1(F74G)
UseTagsExpressionMammalianMutationPromoterEF1 promoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2
Plasmid#194680PurposeCMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2DepositorInsertHpep1-betaArrestin2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMK395 (AAVS1 CMV-OsTIR1(F74A))
Plasmid#159992PurposeExpression of OsTIR1(F74A) in human cellsDepositorInsertCMV-OsTIR1(F74A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-NLS-copGFP-EF1-BlastiS
Plasmid#132772PurposeSpills into the cytoplasm during nuclear envelope rupture.DepositorInsertNLS-copGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 CMV sTurboID (C)-Arrestin-GFP
Plasmid#153010PurposeExpresses split-TurboID C-terminal fragment fused to arrestinDepositorInsertsTurboID (C)(G74)-Arrestin
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING V155M
Plasmid#102591PurposeLentivector to express STING V155M and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationV155MPromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK427 (OsTIR1(WT) mAID-EGFP-Nluc)
Plasmid#140658PurposeOsTIR1(WT)-P2A-mAID-EGFP-NlucDepositorInsertOsTIR1(WT) mAID-EGFP-Nluc TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING-342STOP
Plasmid#102588PurposeLentivector to express STING delta 342 and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationdelta 342PromoterSFFVAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)
Plasmid#126580PurposeA repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locusDepositorInsertOsARF16-PB1 domain
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Blasticidin-CAG-Flpe-ERT2
Plasmid#68461PurposeAAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection geneDepositorInsertmammalian-codon-optimized Flpe recombinase fused with ERT2
UseSynthetic BiologyTagsERT2ExpressionMammalianMutationPromoterCAGAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING 368STOP
Plasmid#102590PurposeLentivector to express STING delta 368 and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationdelta 368PromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING-354STOP
Plasmid#102589PurposeLentivector to express STING delta 354 and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationdelta 354PromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
Plasmid#198417PurposeExpresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2D)
Plasmid#29774DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSEM255 - sgRNA mosTI Pmlc-2_GFP
Plasmid#159835PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertsgRNA mosTI Pmlc-2_GFP
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEf1a OsTIR ires NEO pA T2BH
Plasmid#127910PurposeTransposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoterDepositorInsertOsTIR
UseTransposonTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_ST-SBP-EGFP
Plasmid#65262Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Golden-Gate-Esp3I-Destination
Plasmid#51278PurposeCAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3IDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2E)
Plasmid#29775DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 WT
Plasmid#182046Purposeexpression of Sox2DepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin
Plasmid#199585PurposeExpress tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-hygro-STING HAQ+V155M
Plasmid#102601PurposeExpress STING V55M HAQ for IFN-beta Luciferase reporter assay.DepositorInsertSTING (STING1 Human)
UseRetroviralTagsExpressionMammalianMutationV155M R71H-G230A-R293QPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA
Plasmid#133339Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-STING-C64S,C65S-FLAG
Plasmid#198186PurposeMammalian expression of FLAG-tagged mouse STING1 C64S, C65SDepositorInsertTMEM173 (Sting1 Mouse)
UseTagsFLAGExpressionMammalianMutationThe cysteine in STING at position 64/65 mutates t…PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLOC CMV-IRES-Dsred-blasticidin
Plasmid#129428Purposeexpression of Dsred-blasticidin under CMVpromoter -IRESDepositorInsertDsred-blasticidin
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only