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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 442 results for: 1495

Showing: 181 - 200 of 442 results

  1. pPD95_77

    Plasmid
    #1495
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, rf2204, gfpN, IVSR, gfpS65C, IVSS, IVST, gf…
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1495-plasmid-map-sequence-id-106

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  1. RAB14 S25N

    Plasmid
    #61495
    Purpose
    to overexpress dominant negative RAB14. Vector includes GFP driven by hUbC promoter
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    RAB14 (RAB14 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    S25N, inactive form
    Promoter
    EF1a
    Available Since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pcDNA3-Dronpa-Kras

    Plasmid
    #182329
    Purpose
    Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Dronpa-Kras
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    182329-plasmid-map-sequence-id-363283

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  7. pDronpa-N1-Tau-μDpTT

    Plasmid
    #182323
    Purpose
    Microtubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Tau-μDronpa-TagRFP-T
    Use
    Tags
    MAP tau
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182323-plasmid-map-sequence-id-363424

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  9. pLSLZ

    Plasmid
    #51495
    Purpose
    pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    Zif268:FokI
    3kb filler sequence
    Use
    Plant t-dna plasmid
    Tags
    SV40 NLS
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51495-plasmid-map-sequence-id-78592

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  10. RIB VP64 R3

    Plasmid
    #161495
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R3_gRNA_MS2
    Depositor
    Diethard Mattanovich, Brigitte Gasser
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Targeting R3 in the RIB1 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGAP, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161495-plasmid-map-sequence-id-326166

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  13. pcDNA3-DAKAP-μDpTT

    Plasmid
    #182324
    Purpose
    Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DAKAP-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal 30 amino acids from DAKAP1
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182324-plasmid-map-sequence-id-362788

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  14. pcDNA3-Lyn-μDpTT

    Plasmid
    #182321
    Purpose
    Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Lyn-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182321-plasmid-map-sequence-id-362887

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  15. pcDNA3-CYP450-μDpTT

    Plasmid
    #182325
    Purpose
    Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DAKAP-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal 27 amino acids from CYP450
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182325-plasmid-map-sequence-id-362816

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  16. pcDNA3-Lifeact-μDpTT

    Plasmid
    #182322
    Purpose
    Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Lifeact-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal 17 amino acids from budding yeast Abp1…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182322-plasmid-map-sequence-id-362772

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  18. pcDNA3-DrFLINC-AKAR-Kras

    Plasmid
    #182334
    Purpose
    Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DrFLINC-AKAR
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    182334-plasmid-map-sequence-id-363289

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  20. pcDNA3-H2B-μDpTT

    Plasmid
    #182328
    Purpose
    Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    H2B-μDronpa-TagRFP-T (H2BC3 Human)
    Use
    Tags
    Full-length human histone H2B
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    182328-plasmid-map-sequence-id-363396

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Showing: 181 - 200 of 442 results