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pET21a(+)-HpDsbG C162A

Plasmid

#39330

Depositor

SeWon Suh

Article

Yoon et al FEBS Lett. 2011 Dec 15;585(24):3862-7. E

Insert

oxidoreductase

Tags

His-tag

Expression

Bacterial

Mutation

changed Cysteine 162 to Alanine

Promoter

T7

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pGWT35S-mCherry-NLS

Plasmid

#194049

Purpose

Transient expression of mCherry-NLS in plant cell (Nucleus)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

mCherry

Tags

SV40 (Nuclear localization signal)

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA351-Cpf1

Plasmid

#192361

Purpose

CRISPR-based vector for genome editing

Depositor

Juana María Navarro Llorens

Article

Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

Insert

Cas12a, gRNA scaffold

Use

CRISPR

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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p15A_SpyCas9_CmR

Plasmid

#119162

Purpose

Expresses S. pyogenes Cas9

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

Cas9 from S. pyogenes (NEWENTRY )

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPerFLEX

Plasmid

#217569

Purpose

Bacterial expression of HyPerFLEX

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX

Tags

6xHis

Expression

Bacterial

Promoter

T5

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mNeonGreen

Plasmid

#191743

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mNeonGreen

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mTagBFP2

Plasmid

#191746

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mTagBFP2

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_vsfGFP9

Plasmid

#191747

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and vsfGFP-9

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Cerulean-SKL

Plasmid

#194048

Purpose

Transient expression of Cerulean-SKL in plant cell (Peroxisome)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

Cerulean

Tags

Ser-Lsy-Leu

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)-HpENR

Plasmid

#12651

Depositor

SeWon Suh

Article

Lee et al Acta Crystallogr D Biol Crystallogr. 200

Insert

noyl-acyl carrier protein reductase (fabI Helicobacter pylori)

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_iRFP670

Plasmid

#191749

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and iRFP670

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mScarlet

Plasmid

#191745

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mScarlet

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mApple

Plasmid

#191741

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mApple

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mCherry

Plasmid

#191742

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mCherry

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLG017_Avs1a(I134K)

Plasmid

#248945

Purpose

AVAST from E. piriflorinigrans with Avs1a mutated (I134K)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

Avs1a(I134K)

Expression

Bacterial

Mutation

I134K

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLG017_Avs1a(I234K)

Plasmid

#248946

Purpose

AVAST from E. piriflorinigrans with Avs1a mutated (I234K)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

Avs1a(I234K)

Expression

Bacterial

Mutation

I234K

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLG017_FLAG-Avs1a

Plasmid

#248948

Purpose

AVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

FLAG-Avs1a

Tags

3xFLAG

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLG017_Avs1a_chimera

Plasmid

#248950

Purpose

AVAST from E. piriflorinigrans with Avs1a replaced with Avs1a from Citrobacter Avs1a

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

Avs1a_chimera

Expression

Bacterial

Mutation

I134K,I234K

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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HULAa30F3

Plasmid

#179620

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa30F3

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pPt_Sap

Plasmid

#206856

Purpose

A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome.

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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