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Plasmid#194050PurposeTransient expression of VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Cytosol)DepositorInsertVN154 (mVenus β-strands 1 to 7, amino acids 1−154)
UseTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO5(wt)
Plasmid#191803PurposeFull length wt AtPCO5 with Nt-His tagDepositorInsertAtPCO5
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO1(wt)
Plasmid#191798PurposeFull length wt AtPCO1 with Nt-His tagDepositorInsertAtPCO1
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO2(wt)
Plasmid#191799PurposeFull length wt AtPCO2 with Nt-His tagDepositorInsertAtPCO2
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO3(wt)
Plasmid#191800PurposeFull length wt AtPCO3 with Nt-His tagDepositorInsertAtPCO3
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAC16
Plasmid#137965PurposeExpresses Zea mays FNR and Prochlorococcus marinus NATL2A SIRDepositorInsertsZea mays Ferredoxin-NADP reductase
Procholoroccus marinus NATL2A sulfite reductase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSAC17
Plasmid#137966PurposeExpresses Zea mays FNR and Prochlorococcus marinus MIT9211 SIRDepositorInsertsZea mays Ferredoxin-NADP reductase
Procholoroccus marinus MIT9211 sulfite reductase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-JHP933
Plasmid#59302DepositorInsertjhp0933
UseTagsHis tagExpressionBacterialMutationaa1-232PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVDL9.3
Plasmid#168299PurposeSecretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyADepositorInsertSegment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
UseTagsExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
mbYFPQS
Plasmid#80742Purposemembrane-targeted biosensor for monitoring changes in intracellular chlorideDepositorInsertYFPQS
UseTagspalmitoylation signal sequence of neuromodulinExpressionMammalianMutationH148Q (CAC to CAG) and V163S (GTG to AGC).PromoterCMVAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-GFP
Plasmid#100009PurposeExpresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneomeDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-GFPExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR
Plasmid#100008PurposeExpresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genomeDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2
Plasmid#191751PurposeMicrotubule marker together with nuclear envelope marker driven by bidirectional promoterDepositorUseTagsGdp1 promoter, ToxA promoter, iRFP670, and mTagBF…ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMALp2x-SCO5461
Plasmid#42507DepositorInsertguanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
UseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
CtkA_full_cmv-10
Plasmid#27433DepositorInsertCell translocating kinas A (jhp0940 H. pylori)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
CtkA_300_cmv-10
Plasmid#27434DepositorInsertCell translocating kinas A (jhp0940 H. pylori)
UseTags3xFLAGExpressionMammalianMutationresidues 301-325 are truncatedPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
CtkA_D155Q/D179Q_cmv-10
Plasmid#27435DepositorInsertCell translocating kinas A (jhp0940 H. pylori)
UseTags3xFLAGExpressionMammalianMutationD155Q/D179QPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
CtkA_300_pET21a
Plasmid#27429DepositorInsertCell translocating kinas A (jhp0940 H. pylori)
UseTagsHisExpressionBacterialMutationresidues 301-325 are truncatedPromoterAvailable sinceMay 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
CtkA_D155Q_pET21a
Plasmid#27430DepositorInsertCell translocating kinas A (jhp0940 H. pylori)
UseTagsHisExpressionBacterialMutationD155QPromoterAvailable sinceMay 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
CtkA_D179Q_pET21a
Plasmid#27431DepositorInsertCell translocating kinas A (jhp0940 H. pylori)
UseTagsHisExpressionBacterialMutationD179QPromoterAvailable sinceMay 2, 2011AvailabilityAcademic Institutions and Nonprofits only