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pFUSE-rIgG-Fc2-ICAM1

Plasmid

#156462

Purpose

Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

Depositor

Dirk Mielenz

Article

Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

Insert

Intercellular Adhesion Molecule 1 (Icam1 Mouse)

Tags

ICAM1-rFc

Expression

Bacterial and Mammalian

Mutation

endogenous signal peptide replaced with IL2 signa…

Promoter

hEF1-HTLC

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_short (JDW 1046)

Plasmid

#224480

Purpose

Gateway compatible 5' entry clone containing the Human minimal ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

min-ICAM2 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA1

Plasmid

#99734

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-Dual-iCas9-hALB

Plasmid

#167502

Purpose

Expression of dual inducible Cas9 with hALB promoter

Depositor

Jake Liang

Article

Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

Cas9

Use

CRISPR, Lentiviral, and Luciferase

Tags

FLAG

Expression

Mammalian

Promoter

hALB

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag17-iCAM-1

Plasmid

#205204

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG17

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag17 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA2

Plasmid

#99735

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CHICA (Nigg HS342)

Plasmid

#41153

Depositor

Erich Nigg

Article

Santamaria et al Curr Biol. 2008 May 20;18(10):723-9.

Insert

CHICA (FAM83D Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

V11L

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-SauriCas9-Puro

Plasmid

#135965

Purpose

Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_full (JDW 1047)

Plasmid

#224479

Purpose

Gateway compatible 5' entry clone containing the Human ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

ICAM2 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag6-iCAM-1

Plasmid

#205210

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG6

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag6 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag18-iCAM-1

Plasmid

#205207

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG18

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag18 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag42-iCAM-1

Plasmid

#205205

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG42

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag42 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pColdII-SIRPT1-Loxodonta Africana

Plasmid

#75351

Purpose

Nt His-tag Loxodonta Africana 89-1368

Depositor

Kalle Gehring

Insert

Sacsin Loxodonta Africana 89-1368

Tags

His-Tag

Expression

Bacterial

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-RGDSP-MMP

Plasmid

#112633

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-RGDSP-MMP

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

central matrix metalloproteinase-cleavable sequen…

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Brevican [N294A/10R]

Plasmid

#177528

Purpose

Mammalian Expression Plasmid of anti-Brevican (Rat). Derived from hybridoma N294A/10R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Brevican (Rattus norvegicus) recombinant mouse monoclonal antibody (Bcan Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Versican [N351/23R]

Plasmid

#128639

Purpose

Mammalian Expression Plasmid of anti-Versican (Mouse). Derived from hybridoma N351/23.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Versican (Mouse) recombinant mouse monoclonal antibody (Vcan Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA3

Plasmid

#99736

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-hCA4-IV68

Plasmid

#244907

Purpose

Rep/Cap plasmid for producing variant AAV-hCA4-IV68

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

Synthetic construct producing AAV REP/CAP

Use

AAV

Expression

Mammalian

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-hCA4-IV77

Plasmid

#244906

Purpose

Rep/Cap plasmid for producing variant AAV-hCA4-IV77

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

Synthetic construct producing AAV REP/CAP

Use

AAV

Expression

Mammalian

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

Plasmid

#244844

Purpose

Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

iCas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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