20,043 results

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pHM6-alphasynuclein-WT

Plasmid

#40824

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Tags

6x His and HA

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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V5-GFP-TurboID-KDEL_pLX208

Plasmid

#202020

Purpose

expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-TurboID-KDEL

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA Scramble

Plasmid

#162011

Purpose

Lentiviral negative control vector containing scramble shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

scramble shRNA

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake3

Plasmid

#139342

Purpose

Expresses Curlcake3 (CC3) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake3 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake1

Plasmid

#139340

Purpose

Expresses Curlcake1 (CC1) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake1 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake2

Plasmid

#139341

Purpose

Expresses Curlcake2 (CC2) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake2 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake4

Plasmid

#139343

Purpose

Expresses Curlcake4 (CC4) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake4 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSFV3-lacZ

Plasmid

#92074

Purpose

This is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Insert

lacZ

Use

Lacz expression plasmid, for run-off transcriptio…

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-pBAD

Plasmid

#54572

Purpose

Localization: Protein Expression Vector , Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis, T7, and mTagBFP2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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sox10:mCherry

Plasmid

#98695

Purpose

I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

mCherry

Use

Zebrafish expression

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCW empty vector

Plasmid

#184708

Purpose

Lentiviral empty vector for doxycycline-inducible expression

Depositor

Alessia Ciarrocchi, Gloria Manzotti

Article

Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pQE30-His-T7RNAP

Plasmid

#124138

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

T7 RNA polymerase (T7p07 Enterobacteria phage T7)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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K44A HA-dynamin 2 pcDNA3.1

Plasmid

#34685

Depositor

Sandra Schmid

Insert

dynamin 2ba

Tags

HA (variant)

Expression

Mammalian

Mutation

K44A

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-ER Y537S

Plasmid

#49499

Purpose

Expresses HA-tagged ER Y537S mutant in mammalian cells

Depositor

Sarat Chandarlapaty

Article

Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

Insert

ESR1 (ESR1 Human)

Tags

HA

Expression

Mammalian

Mutation

Tyrosine 537 to Serine

Promoter

CMV

Available Since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3 2 kb prom. CD274

Plasmid

#107003

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p65-mNeonGreen

Plasmid

#127172

Purpose

Fluorescently tagged p65 reporter

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

GFP-RelA (RELA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCE-GFP

Plasmid

#41858

Purpose

Non-integrating (episomal) expression of EGFP

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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