9,096 results

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pCMV-HsPeg10rc3

Plasmid

#174859

Purpose

Expresses recoded human Peg10 in mammalian cells

Depositor

Feng Zhang

Publication

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

recoded Homo sapiens PEG10 (PEG10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-KRAB-MeCP2

Plasmid

#122267

Purpose

SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

Depositor

Andrea Califano

Publication

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Tags

dCas9-KRAB-MeCP2

Expression

Mammalian

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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p2CT-His-MBP-Lbu_C2c2_WT

Plasmid

#83482

Purpose

Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Publication

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEvol-pAzFRS.2.t1

Plasmid

#73546

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Publication

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.2.t1
pAzFRS.2.t1

Expression

Bacterial

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCBC-DT2T3

Plasmid

#50591

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Publication

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T2, gRNA scaffold, U6-29t plus, U6-1p, T3

Use

CRISPR; Pcr template

Expression

Plant

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLV hUbC-VP64 dCas9 VP64-T2A-GFP

Plasmid

#59791

Purpose

Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized VP64 dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pJFRC81-10XUAS-IVS-Syn21-GFP-p10

Plasmid

#36432

Depositor

Gerald Rubin

Publication

Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

Insert

GFP

Expression

Insect

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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pT7-EGFP-C1-HsDCP1a

Plasmid

#25030

Depositor

Elisa Izaurralde

Publication

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP1a (DCP1A Human)

Tags

EGFP

Expression

Mammalian

Available since

June 23, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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p2xFlag CMV2-YAP2

Plasmid

#19045

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pWZL Blast Twist ER

Plasmid

#18799

Depositor

Bob Weinberg

Publication

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

twist (Twist1 Mouse)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Klotho (membrane)

Plasmid

#17712

Depositor

Hal Dietz

Publication

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Tags

V5

Expression

Mammalian

Mutation

P101S

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pMIG-hKLF4

Plasmid

#17227

Depositor

George Daley

Publication

Park et al Nature. 2008 Jan 10. 451(7175):141-6.

Insert

Kruppel-like factor 4 (gut) (KLF4 Human)

Use

Retroviral

Expression

Mammalian

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Publication

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pGP-FF

Plasmid

#13480

Depositor

Keith Joung

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Type

Empty backbone

Tags

Gal11P

Expression

Bacterial

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-SHP2 WT

Plasmid

#8381

Depositor

Ben Neel

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Available since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pmScarlet_Giantin_C1

Plasmid

#85048

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pLV-TetO-hNGN2-Puro

Plasmid

#79049

Purpose

Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Publication

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pMXs-puro-RFP-ATG9A

Plasmid

#60609

Purpose

Expresses RFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Publication

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

RFP

Expression

Mammalian

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GFP-Rab7A

Plasmid

#61803

Purpose

Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Publication

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

Rab7A (RAB7A Human)

Tags

2x HA and GFP

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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