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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,358 results for: BLI;

Showing: 3961 - 4000 of 8358 results

  1. pmScarlet3-H_I3

    Plasmid
    #239343
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Torsten Wittmann
    Insert
    I3-01
    Use
    Tags
    mScarlet3-H (a.k.a. mYongHong)
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239343-plasmid-map-sequence-id-474361

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  2. pmStayGold(E138D)_I3

    Plasmid
    #239338
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold(E138D) in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Torsten Wittmann
    Insert
    I3-01
    Use
    Tags
    mStayGold(E138D)
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239338-plasmid-map-sequence-id-474341

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  3. pBOBI-hPLD4-flag

    Plasmid
    #222641
    Purpose
    lentiviral vector for full length human PLD4 expression (with flag tag)
    Depositor
    David Nemazee
    Insert
    PLD4 (PLD4 Human)
    Use
    Lentiviral
    Tags
    flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    222641-plasmid-map-sequence-id-472419

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  4. LLhKdgR-pHG165a 2mut

    Plasmid
    #220270
    Purpose
    Constitutive expression of a chimeric LacI:KdgR transcription repressor
    Depositor
    Liskin Swint-Kruse
    Insert
    LLhKdgR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V52A/Q55V
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    220270-plasmid-map-sequence-id-436464

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  5. LLhRafR-pHG165a G62A

    Plasmid
    #220273
    Purpose
    Constitutive expression of a chimeric LacI:RafR transcription repressor
    Depositor
    Liskin Swint-Kruse
    Insert
    LLhRafR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G62A
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    220273-plasmid-map-sequence-id-436467

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  6. LLhRafR-pHG165a 3mut

    Plasmid
    #220274
    Purpose
    Constitutive expression of a chimeric LacI:RafR transcription repressor
    Depositor
    Liskin Swint-Kruse
    Insert
    LLhRafR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V52N/Q55L/Q62H
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    220274-plasmid-map-sequence-id-436468

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  7. LLhSalR-pHG165a 3mut

    Plasmid
    #220276
    Purpose
    Constitutive expression of a chimeric LacI:SalR transcription repressor
    Depositor
    Liskin Swint-Kruse
    Insert
    LLhSalR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V52L/Q55G/Y62R
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    220276-plasmid-map-sequence-id-436470

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  8. LLhScrR-pHG165a 3mut

    Plasmid
    #220278
    Purpose
    Constitutive expression of a chimeric LacI:ScrR transcription repressor
    Depositor
    Liskin Swint-Kruse
    Insert
    LLhScrR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V52I/Q62S/G120S
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    220278-plasmid-map-sequence-id-436472

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  9. EP003: CAG::GEM7-HaloTag

    Plasmid
    #209303
    Purpose
    Expression plasmid for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice via in utero electroporation
    Depositor
    Adam Cohen
    Insert
    GEM7
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    209303-plasmid-map-sequence-id-414428

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  10. EP004: pAAV TRE::GEM7-HaloTag

    Plasmid
    #209304
    Purpose
    Doxycycline-gated expression plasmid (TetOFF) for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice
    Depositor
    Adam Cohen
    Insert
    GEM7
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    209304-plasmid-map-sequence-id-414377

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  11. pAN68

    Plasmid
    #164525
    Purpose
    Retroviral expression of CENP-R uORF-1
    Depositor
    Iain Cheeseman
    Insert
    CENP-R uORF-1 (ITGB3BP Human)
    Use
    Retroviral
    Tags
    -6xhis-precission-GFP
    Expression
    Mutation
    Promoter
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    164525-plasmid-map-sequence-id-328004

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  12. pAG Retro EGFP Neo

    Plasmid
    #234464
    Purpose
    Control retroviral vector expressing Green Fluorescent Protein. Neomycin selection.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Green Fluorescent Protein
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234464-plasmid-map-sequence-id-464634

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  13. pCS2-zH2B-GFP

    Plasmid
    #226561
    Purpose
    Express H2B-GFP in zebrafish.
    Depositor
    Yong Zhang
    Insert
    h2b (h2bc1 Zebrafish)
    Use
    Synthetic Biology; Zebrafish expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    226561-plasmid-map-sequence-id-461800

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  14. pAIP1

    Plasmid
    #233685
    Purpose
    Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.
    Depositor
    Christopher Bahl
    Insert
    Ulp1-like SUMO protease
    Use
    Tags
    CAHS-107838 and HisTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 4, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233685-plasmid-map-sequence-id-463803

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  15. pZP05

    Plasmid
    #232319
    Purpose
    pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum
    Depositor
    Chenggang Wu
    Insert
    Ori-repA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232319-plasmid-map-sequence-id-462197

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  16. 3'dmDR-CMV-CasRx-bGHpolyA

    Plasmid
    #229556
    Purpose
    Expresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' end
    Depositor
    Omar Akbari
    Insert
    CasRx
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229556-plasmid-map-sequence-id-455012

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  17. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

    Plasmid
    #221448
    Purpose
    Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3
    Depositor
    Omar Akbari
    Inserts
    wtCas9-NG
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221448-plasmid-map-sequence-id-437819

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  18. pLaG2-T7RNAPC

    Plasmid
    #216337
    Purpose
    Arabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAP
    Depositor
    Mark Styczynski
    Insert
    LaG2-T7RNAPC fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    g2658a, g3915a
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    216337-plasmid-map-sequence-id-456751

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  19. pBS-ACTB-C200-T7

    Plasmid
    #229850
    Purpose
    Donor vector for the ACTB site containing T7 sequence for detection of HR products.
    Depositor
    Natsuko Chiba
    Insert
    Partial sequence of ACTB gene
    Use
    Hr donor vector for human actb gene.
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    229850-plasmid-map-sequence-id-457516

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  20. pCG-scr

    Plasmid
    #229847
    Purpose
    Expresses wild-type Cas9 and scrambled gRNA.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA with scrambled sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    229847-plasmid-map-sequence-id-457515

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  21. pDual_dsCas9_Venus_hs_NC_chr1

    Plasmid
    #214682
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214682-plasmid-map-sequence-id-428816

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  22. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid
    #214683
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214683-plasmid-map-sequence-id-428804

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  23. pDual_dsCas9_Venus_hs_TP73

    Plasmid
    #214685
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214685-plasmid-map-sequence-id-428818

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  24. pDual_dsCas9_BFP_hs_NC_chr1

    Plasmid
    #214686
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214686-plasmid-map-sequence-id-428810

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  25. pDual_dsCas9_BFP_hs_TP73

    Plasmid
    #214687
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214687-plasmid-map-sequence-id-428806

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  26. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

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  27. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

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  28. pHR-TRE-miRFP703_PGK-Puro

    Plasmid
    #216667
    Purpose
    Expresses tTa-inducible miRFP703, with puromycin resistance
    Depositor
    Leonardo Morsut
    Inserts
    miRFP703
    PuroR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216667-plasmid-map-sequence-id-432197

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  30. pAAV-phSyn-flex-SypHaloTag

    Plasmid
    #172280
    Purpose
    Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.
    Depositor
    Danny Winder
    Insert
    synaptophysin-HaloTag
    Use
    AAV and Cre/Lox
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    rat synapsin
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    172280-plasmid-map-sequence-id-337805

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  31. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Use
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

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  32. pIND4-GFP-MmXYRS

    Plasmid
    #222745
    Purpose
    Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Steven Boxer
    Inserts
    sfGFP
    MmXYRS
    Mm Pyl tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222745-plasmid-map-sequence-id-442565

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  33. pGEX-6p1-GST-Ubiquitin

    Plasmid
    #223236
    Purpose
    Bacterial expression construct for GST-Tagged Ubiquitin production and purification
    Depositor
    Weixing Zhao
    Insert
    Ubiquitin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223236-plasmid-map-sequence-id-443802

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  34. H2B-Electra2

    Plasmid
    #218929
    Purpose
    Electra2 fused to human H2B protein for nuclear localization
    Depositor
    Amro Hamdoun
    Insert
    H2B-Electra2 (H2B Human, Entacmaea quadricolor, Synthetic)
    Use
    Synthetic Biology
    Tags
    Electra2 and H2B
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218929-plasmid-map-sequence-id-433882

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  35. LvPolyUb

    Plasmid
    #218982
    Purpose
    L.variegatus polyubiquitin-C promoter
    Depositor
    Amro Hamdoun
    Insert
    L.variegatus polyubiquitinC promoter
    Use
    Sea urchin
    Tags
    Expression
    Mutation
    Promoter
    L.variegatus polyubiquitinC promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218982-plasmid-map-sequence-id-433881

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  36. pet28a+_Yjjg

    Plasmid
    #220228
    Purpose
    Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    220228-plasmid-map-sequence-id-439539

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  37. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

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  38. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218395-plasmid-map-sequence-id-433911

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  39. 1167A

    Plasmid
    #218233
    Purpose
    HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene
    Depositor
    Omar Akbari
    Insert
    putative metabolite transport protein
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    218233-plasmid-map-sequence-id-433264

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  40. pET28-Flag-RBM24

    Plasmid
    #208621
    Purpose
    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208621-plasmid-map-sequence-id-433637

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Showing: 3961 - 4000 of 8358 results