We narrowed to 8,206 results for: Dos
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Plasmid#226546PurposeBacterial expression of N-terminally 6His tagged A1-LCD X4DepositorInsertA1-LCD swap X4
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swapX3
Plasmid#226545PurposeBacterial expression of N-terminally 6His tagged A1-LCD X3DepositorInsertA1-LCD swap X3
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap V4
Plasmid#226541PurposeBacterial expression of N-terminally 6His tagged A1-LCD V4DepositorInsertA1-LCD swap V4
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap X10
Plasmid#226552PurposeBacterial expression of N-terminally 6His tagged A1-LCD X10DepositorInsertA1-LCD swap X10
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap X9
Plasmid#226551PurposeBacterial expression of N-terminally 6His tagged A1-LCD X9DepositorInsertA1-LCD swap X9
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap X8
Plasmid#226550PurposeBacterial expression of N-terminally 6His tagged A1-LCD X8DepositorInsertA1-LCD swap X8
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap X6
Plasmid#226548PurposeBacterial expression of N-terminally 6His tagged A1-LCD X6DepositorInsertA1-LCD swap X6
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest17_A1-LCD swap X2
Plasmid#226544PurposeBacterial expression of N-terminally 6His tagged A1-LCD X2DepositorInsertA1-LCD swap X2
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D
Plasmid#215910PurposeExpression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutationDepositorInsertTNRC6A (TNRC6A Human)
TagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1332D, S1346D, S1616D, S169…Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A
Plasmid#215911PurposeExpression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
TagsFlag-HA-SBPExpressionMammalianMutationcontain aa1224-1709, S1332A, S1346A, S1616A, S169…Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A
Plasmid#215905PurposeExpression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
Tagsmyc-GFPExpressionMammalianMutationcontain aa1224-1709, S1332A, S1346A, S1616A, S169…Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSII-FRT-MCS
Plasmid#205988PurposeVector containing FRT and FRT-F3 sitesDepositorTypeEmpty backboneUsePcr vectorAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-3
Plasmid#220440PurposeBase construct used to create the PUF3/PUP2 tagging strain. (aka pCS2/3HA/PUM1_RBD/PUP2/URA3)DepositorTypeEmpty backboneExpressionYeastPromoterMV IE93 promoterAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
RT-1
Plasmid#220434PurposeBase construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)DepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K5Q-GS3
Plasmid#204746PurposeExpression of Cas9 and human H2AX with the K5Q mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K5R-GS3
Plasmid#204747PurposeExpression of Cas9 and human H2AX with the K5R mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134L-GS3
Plasmid#204750PurposeExpression of Cas9 and human H2AX with the K134L mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134M-GS3
Plasmid#204751PurposeExpression of Cas9 and human H2AX with the K134M mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134A-GS3
Plasmid#204752PurposeExpression of Cas9 and human H2AX with the K134A mutationDepositorAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXOOY
Plasmid#196449PurposeA dual vector for expression of membrane proteins in S. cerevisiae and X. laevisDepositorTypeEmpty backboneUseExpression in xenopus laevisExpressionYeastPromoteryeast CYC-GAL and T7 promoterAvailable SinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
K365-iLid
Plasmid#188456PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
Tags6xHis and iLidExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-micro
Plasmid#188457PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
Tags6xHis-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Bmp7
Plasmid#201909Purposein vitro transcription of RNA encoding FLAG-tagged Bmp7aDepositorAvailable SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-sgHPRT1
Plasmid#196713PurposeCRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TGDepositorInsertCas9-T2A-BSD-U6-sgHPRT1
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterEF1a/hU6Available SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7_1-633-shRNAres_U
Plasmid#147645PurposeMammalian Expression of HsSmg7_1-633-shRNAresDepositorInsertHsSmg7_1-633-shRNAres (SMG7 Human)
ExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G co…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7_633-1091-siRNAres_U
Plasmid#147646PurposeMammalian Expression of HsSmg7_633-1091-siRNAresDepositorInsertHsSmg7_633-1091-siRNAres (SMG7 Human)
ExpressionMammalianMutationtwo non silent mutations Q651R and V854I compared…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvG-HsNot8_1-240_U
Plasmid#147648PurposeBacterial Expression of HsNot8_1-240DepositorInsertHsNot8_1-240 (CNOT8 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5_267-1017-V5-SBP-MBP_V
Plasmid#147762PurposeMammalian Expression of HsSmg5_267-1017DepositorInsertHsSmg5_267-1017 (SMG5 Human)
ExpressionMammalianMutationone silent mutation C969T compared to the sequenc…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-del133-155-V5-SBP-MBP_V
Plasmid#147763PurposeMammalian Expression of HsSmg5-del133-155DepositorInsertHsSmg5-del133-155 (SMG5 Human)
ExpressionMammalianMutationone silent mutation C969T compared to the sequenc…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-del267-809-V5-SBP-MBP_V
Plasmid#147764PurposeMammalian Expression of HsSmg5-del267-809DepositorInsertHsSmg5-del267-809 (SMG5 Human)
ExpressionMammalianMutationone silent mutation C969T compared to the sequenc…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-del810-846-V5-SBP-MBP_V
Plasmid#147765PurposeMammalian Expression of HsSmg5-del810-846DepositorInsertHsSmg5-del810-846 (SMG5 Human)
ExpressionMammalianMutationone silent mutation C969T compared to the sequenc…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-del847-1017-V5-SBP-MBP_V
Plasmid#147766PurposeMammalian Expression of HsSmg5-del847-1017DepositorInsertHsSmg5-del847-1017 (SMG5 Human)
ExpressionMammalianMutationone silent mutation C969T compared to the sequenc…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7_1-633_U
Plasmid#147644PurposeMammalian Expression of HsSmg7_1-633DepositorInsertHsSmg7_1-633 (SMG7 Human)
ExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G co…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5-del133-155_T
Plasmid#147553PurposeMammalian Expression of HsSmg5-del133-155DepositorInsertHsSmg5-del133-155 (SMG5 Human)
ExpressionMammalianMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5-del133-155-G120E_T
Plasmid#147554PurposeMammalian Expression of HsSmg5-del133-155-G120EDepositorInsertHsSmg5-del133-155-G120E (SMG5 Human)
ExpressionMammalianMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5-Q123E_T
Plasmid#147555PurposeMammalian Expression of HsSmg5-Q123EDepositorInsertHsSmg5-Q123E (SMG5 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg7_1-633_T
Plasmid#147557PurposeMammalian Expression of HsSmg7_1-633DepositorInsertHsSmg7_1-633 (SMG7 Human)
ExpressionMammalianMutationtwo silent mutation G216A, A1521G compared to the…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-tdTomato-sgRNA
Plasmid#194724Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target tdTomatoDepositorInsertAsCpf1
UseCRISPRExpressionMammalianAvailable SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA
Plasmid#194725Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFBDepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-tdTomato-2A-mSyp::EGFP
Plasmid#191213PurposeExpresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.DepositorAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLTR-DuvSAF
Plasmid#190914PurposePseudotyping of lentiviral vectorsDepositorInsertLyssavirus duvenhage antigenic glycoprotein
ExpressionMammalianPromoterHIV-1 LTRAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsSMG7opt_732-1091_V
Plasmid#147769PurposeBacterial Expression of HsSMG7opt_732-1091DepositorInsertHsSMG7opt_732-1091 (SMG7 Human)
ExpressionBacterialAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3e tdTomato
Plasmid#188700PurposeGateway 3' element encoding tdTomatoDepositorInserttdTomato
UseSynthetic BiologyExpressionMammalianAvailable SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5-G120E_W
Plasmid#147819PurposeMammalian Expression of HsSmg5-G120EDepositorInsertHsSmg5-G120E (SMG5 Human)
ExpressionMammalianMutationone silent mutation compared to the sequence give…Available SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-G120E-del133-155-V5-SBP-MBP_V
Plasmid#147767PurposeMammalian Expression of HsSmg5-G120E-del133-155DepositorInsertHsSmg5-G120E-del133-155 (SMG5 Human)
ExpressionMammalianMutationone silent mutation C969T compared to the sequenc…Available SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvG-HsNot8_13-240_U
Plasmid#147649PurposeBacterial Expression of HsNot8_13-240DepositorInsertHsNot8_13-240 (CNOT8 Human)
ExpressionBacterialAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg7_633-1091_T
Plasmid#147558PurposeMammalian Expression of HsSmg7_633-1091DepositorInsertHsSmg7_633-1091 (SMG7 Human)
ExpressionMammalianMutationone non silent mutation V854I compared to the seq…Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-197
Plasmid#169924PurposeWT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2DepositorInsertEcm2 1-197
ExpressionBacterial and YeastAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEL2004
Plasmid#139889PurposeExpression plasmid for LetAB with 6 ring (rings 123457) LetB (without TM region) mutantDepositorInsertLetB
ExpressionBacterialMutationLetB(43-877; delta634-746)-6xHisAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-17
Plasmid#160717PurposeTrpC Terminator bordered by BsaI restriction sitesDepositorInserttryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites
UseSynthetic BiologyMutationIncorporation of BsaI restriction sitesAvailable SinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only