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We narrowed to 7,692 results for: Lif;

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Showing: 4951 - 5000 of 7692 results

  1. ERrefGFP1_pCDNA3

    Plasmid
    #48635
    Purpose
    Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERrefGFP1
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    C147S,C204Q
    Available Since
    Oct. 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    48635-plasmid-map-sequence-id-70401

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  3. As3 (pBW411J117-arsR)

    Plasmid
    #123142
    Purpose
    An arsenic sensor with optimized intracellular receptor density. Also used in the sensor cell array. pSB3K3 carrying J117-30arsR-t-ParsR-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30gfp-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J117, ParsR
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123142-plasmid-map-sequence-id-241980

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  8. As1

    Plasmid
    #123159
    Purpose
    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J117, ParsR, PhrpLE
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123159-plasmid-map-sequence-id-241970

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  9. roGFP_iE_C204S_pQE30

    Plasmid
    #48632
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, c204S
    Available Since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48632-plasmid-map-sequence-id-70381

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  10. pIhh715bp-Luc

    Plasmid
    #53606
    Purpose
    Reporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays
    Depositor
    Xiangli Yang
    Article
    Wang et al Development. 2009 Dec;136(24):4143-53. d
    Insert
    Ihh promoter
    Use
    Luciferase; Reporter
    Available Since
    June 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. p236 pPr-CREM

    Plasmid
    #8404
    Purpose
    Expression of Cre from tissue-specific, androgen-inducible probasin promoter, with chimeric/β-globin intron within the Cre coding sequence.
    Depositor
    Jeffrey Green
    Article
    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56
    Insert
    probasin driven CREM
    Use
    Cre/Lox
    Expression
    Mammalian
    Mutation
    modified Cre
    Promoter
    Probasin promoter
    Available Since
    April 28, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. pJH124

    Plasmid
    #48259
    Purpose
    Template plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    Ag TEF1 promoter
    Use
    Routine cloning vector
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    Ag TEF1 promoter
    Available Since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    48259-plasmid-map-sequence-id-69693

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  21. ADEPT-pTarget-GFP

    Plasmid
    #238041
    Purpose
    Encodes sfGFP under lac promoter. Expresses a single guide RNA (under Rha promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    sfGFP
    Use
    Synthetic Biology
    Promoter
    lac
    Available Since
    June 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    238041-plasmid-map-sequence-id-476072

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  22. ADEPT-pTarget-RFP

    Plasmid
    #238040
    Purpose
    Encodes mRFP1 under pLux promoter. Expresses a single guide RNA (under Ara promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    mRFP1
    Use
    Synthetic Biology
    Promoter
    Lux
    Available Since
    June 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    238040-plasmid-map-sequence-id-476070

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  24. ADEPT-pTarget

    Plasmid
    #238033
    Purpose
    Encodes sfGFP-SsrA under constitutive promoter (J23119). Expresses a single guide RNA, which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    ssrA
    Promoter
    J23119 (constitutive)
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    238033-plasmid-map-sequence-id-471747

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  29. AAV-hSyn-IvfChr-citrine-KV2.1

    Plasmid
    #221615
    Purpose
    A recombinant AAV2 plasmid encoding vfChrimson-citrine with trafficking enhancement and soma targeting with KV2.1 motif.
    Depositor
    John Lin
    Article
    Mermet-Joret et al eLife 2023 12:RP90327
    Insert
    vfChrimson-citrine with membrane trafficking enhancement and soma targeting
    Use
    AAV
    Mutation
    vfChrimson-citrine with membrane trafficking enha…
    Promoter
    Human Synapsin
    Available Since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221615-plasmid-map-sequence-id-442201

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  30. pET28Man

    Plasmid
    #203399
    Purpose
    exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes
    Depositor
    David Vocadlo
    Article
    Deng et al Bioorg Med Chem. 2013 Aug 15;21(16):4839
    Insert
    man2A
    Tags
    H6
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available Since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    203399-plasmid-map-sequence-id-404714

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  32. pITEdv1

    Plasmid
    #218283
    Purpose
    Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218283-plasmid-map-sequence-id-433685

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  33. pITEu2

    Plasmid
    #218282
    Purpose
    Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218282-plasmid-map-sequence-id-433684

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  34. pJE13HD7

    Plasmid
    #218281
    Purpose
    Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218281-plasmid-map-sequence-id-433683

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  35. pSSA-DsRed/eGFP (pXK-442)

    Plasmid
    #208827
    Purpose
    The SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208827-plasmid-map-sequence-id-417251

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  36. pSSA-eGFP (pXK-221)

    Plasmid
    #208826
    Purpose
    The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208826-plasmid-map-sequence-id-417242

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  40. pOPO812

    Plasmid
    #195306
    Purpose
    pBAD322-MycaIDCas-ΔCas6-RGR-lacZs, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked lacZ spacer 5.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade Δcas6 and ribozyme flanked lacZ spacer 5
    Expression
    Bacterial
    Promoter
    Arabinose inducible arapBAD
    Available Since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195306-plasmid-map-sequence-id-383143

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  41. pOPO646

    Plasmid
    #195307
    Purpose
    pBAD322-MycaTnsD, arapBAD promoter expressing cyanobacterial tRNA-Leu gene targeting TnsD protein of McCAST (Tn7575).
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    TnsD of McCAST
    Expression
    Bacterial
    Promoter
    Arabinose inducible arapBAD
    Available Since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195307-plasmid-map-sequence-id-383139

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  47. pSCL.040

    Plasmid
    #184974
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184974-plasmid-map-sequence-id-376705

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  49. pSCL.109

    Plasmid
    #184978
    Purpose
    Test effect of extending a1/a2 on LYP1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    LYP1 donor E27stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184978-plasmid-map-sequence-id-376679

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  50. pSCL.107

    Plasmid
    #184976
    Purpose
    Test effect of extending a1/a2 on CAN1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    CAN1 donor G444stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184976-plasmid-map-sequence-id-376676

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Showing: 4951 - 5000 of 7692 results