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Addgene
Showing: 1981 - 2000 of 151104 results
  1. pDEST17-His10-Smt3-ccdB-SNAP

    Plasmid
    #166148
    Purpose
    Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His10-Smt3 and SNAP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pDEST-Spe4-Kozak-emiRFP703-RfA

    Plasmid
    #186376
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

    Plasmid
    #197454
    Purpose
    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Insert
    FBW7 Delta-WD40 propeller (FBXW7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW& dimerization domain deleted.
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pDEST-Spe4-RfA-mVenus-stop

    Plasmid
    #186394
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mVenus into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pDEST-Spe4-Kozak-miRFP713-RfA

    Plasmid
    #186381
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pDEST-Spe4-Kozak-Halotag-RfA

    Plasmid
    #186377
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3.2-V5-DEST-hSFRP1(aa53-166)

    Plasmid
    #189544
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-166.
    Depositor
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    contains aa53-166
    Promoter
    Available since
    April 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pDEST27-GST-hSFRP1(aa181-314)

    Plasmid
    #189548
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.
    Depositor
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa181-314
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pDEST27-GST-hSFRP1(aa53-169)

    Plasmid
    #189545
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.
    Depositor
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa53-169
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 1981 - 2000 of 151104 results