Addgene

Showing: 2001 - 2020 of 2899 results

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OR4-consensus

Plasmid

#208061

Purpose

Consensus OR4 engineered from human OR4 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR4-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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OR52-consensus

Plasmid

#208080

Purpose

Consensus OR52 engineered from human OR52 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR52-consensus

Use

Tags

Rhodopsin tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RO1.7-dnHIF

Plasmid

#206343

Purpose

AAV plasmid expressing dominant negative HIF1A in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

Insert

dominant negative hif1a

Use

AAV

Tags

Expression

Mutation

dominant negative HIF1A

Promoter

1.7kb human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RedO-nt.Txnip(C247S)(1-301aa)

Plasmid

#206354

Purpose

AAV plasmid expressing deltion and mutant TXNIP in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Tags

Expression

Mutation

(1-301aa), C247S

Promoter

human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pFsynW SYT1deltaC2AB reporter

Plasmid

#197279

Purpose

Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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mCh-Miro1(∆TM)-SspB

Plasmid

#194007

Purpose

Optogenetic tool

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Miro1 (var4, peroxisomal splice variant) (RHOT1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJH4140

Plasmid

#191479

Purpose

Pacr-2s-loxP::EBFP::loxP::Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Chrimson

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pacr-2s

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3557

Plasmid

#191444

Purpose

Pttr-39-Chrimson::wCherry unc-54 3' UTR C.elegans D motor neurou expression of Chrimson RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Chrimson

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pttr-39

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3650

Plasmid

#191446

Purpose

Pacr-2s-Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression of Chrimson RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Chrimson

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pacr-2s

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJH4211

Plasmid

#191532

Purpose

Pacr-2s-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans A/B MN expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GtACR2

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pacr-2s

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[WIV1 RBD]

Plasmid

#190087

Purpose

express chimeric SARS-CoV spike with the WIV-1 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[Khosta2 RBD]

Plasmid

#190083

Purpose

express chimeric SARS-CoV spike with the Khosta-2 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[Khosta1 RBD]

Plasmid

#190082

Purpose

express chimeric SARS-CoV spike with the Khosta-1 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[BtKY72 RBD]

Plasmid

#190081

Purpose

express chimeric SARS-CoV spike with the BtKY72 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[PRD0038 RBD]

Plasmid

#190085

Purpose

express chimeric SARS-CoV spike with the PRD0038 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYn-DIO-ScramblemiR-IRES-mCitrine

Plasmid

#186418

Purpose

Expression of scrambled shRNA used as a control for knock down experiments

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

ScramblemiR

Use

AAV

Tags

mCitrine

Expression

Mutation

Promoter

human Synapsin1

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine

Plasmid

#186419

Purpose

Expression of shRNA for the knock down of mouse Egr3

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

miREgr3-5D

Use

AAV

Tags

mCitrine

Expression

Mutation

Promoter

human Synapsin1

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NLS-QPAS1-VP16

Plasmid

#186187

Purpose

AAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activation

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

Insert

NLS-QPAS1-VP16

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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iLID-mRuby3-iTrkA

Plasmid

#136510

Purpose

Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

iLID mRuby3 iTrkA (Ntrk1 Rat, Avena sativa)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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iLID-mRuby3-iTrkB

Plasmid

#136511

Purpose

Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkB (aa 455-822)

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

iLID mRuby3 iTrkB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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