We narrowed to 8,446 results for: gnal

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pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

Plasmid

#213117

Purpose

Expression of a faster-running MinE mutant in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE(L4E)-EGFP (minE Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Mutation

L4E

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH

Plasmid

#225475

Purpose

Express mEGFP-fusion protein of GAPDH

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

Plasmid

#213116

Purpose

Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

FRB-mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 WT

Plasmid

#111776

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

WT

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLL7.0: tgRFPt-SSPB R73Q

Plasmid

#60416

Purpose

Mammalian Expression of tgRFPt-Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

R73Q, Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-Merlin S518A

Plasmid

#84294

Purpose

Expression of Merlin with an alanine mutation at the Pak phosphorylation site

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S518A

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-PD-L1-SNAP

Plasmid

#223577

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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hE-cadherin/Δp120

Plasmid

#45770

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Δp120 (CDH1 Human)

Expression

Mammalian

Mutation

Amino acids 762-764 converted from EED to AAA to …

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-Merlin S518D

Plasmid

#84295

Purpose

Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S518D

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR(T/A)

Plasmid

#118410

Purpose

Negative-control mutant for ExRai-CKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-isoform a

Plasmid

#225447

Purpose

Express EGFP-fusion protein of FXR1 isoform a

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/EV

Plasmid

#120833

Purpose

Control vector, 20nt random sequence between gateway recombination sites

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

Plasmid

#213118

Purpose

Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

PKAsub-mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab2 S20N

Plasmid

#46780

Purpose

expression of S20N Rab2a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB2 (RAB2A Human)

Tags

myc

Expression

Mammalian

Mutation

S20N dominant negative

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

Plasmid

#225462

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-tdTomato-FlagAkt1

Plasmid

#64831

Purpose

Lentiviral expression of murine Flag-Akt1

Depositor

Eva Gonzalez

Article

Kajno et al Biochem J. 2015 Apr 9.

Insert

Akt1 (Akt1 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

V5-Snail2 (SNAI2 Chicken)

Tags

V5

Expression

Mammalian

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_TRE_Ecad

Plasmid

#133805

Purpose

inducible expression of Ecad in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

Homo sapiens cadherin 1 (CDH1) (CDH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST40-Arl13b11

Plasmid

#40873

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Tags

His and V5

Expression

Mammalian

Mutation

no stop codon

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pDEST40-Arl13b89

Plasmid

#40874

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-GAT3-HA-mCherry

Plasmid

#117722

Purpose

Expresses a GABA transporter GAT-3 (Slc6a11)

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Slc6a11 (Slc6a11 Mouse)

Use

AAV

Tags

HA and mCherry

Expression

Mammalian

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK5

Plasmid

#65244

Purpose

Expression of HA tagged ERK5 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5 (MAPK7 Human)

Tags

HA

Expression

Mammalian

Mutation

A409T

Promoter

CMV

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-gp130dimer

Plasmid

#174394

Purpose

Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain with leucine zipper & C-C linker

Tags

Flag

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-UBE4B

Plasmid

#113499

Purpose

Expression of human UBE4B with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBE4B (UBE4B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST47-Arl13b12

Plasmid

#40872

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Tags

GFP

Expression

Mammalian

Mutation

no stop codon

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRA1 CTFdeltaTA-FLAG

Plasmid

#217711

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRA1 (ADGRA1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-6

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Phi3-GCaMP6f

Plasmid

#106298

Purpose

CMV driven GFP based calcium (Ca2+) reporter

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

GCaMP6f

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-Lckg2a)

Plasmid

#177891

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A

Promoter

Polyhedrin

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pARF1(Q71L)-CFP

Plasmid

#11385

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Changed Glutamine 71 to Leucine.

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pSPAsp-hp

Plasmid

#48122

Purpose

Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

predicted optimal signal peptide for Bacillus megaterium protein secretion

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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