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Plasmid#203482PurposeFor Agrobacterium transformation. tagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertTagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-TP-mCherry-UnaG
Plasmid#203483PurposeFor Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertPlastid transit peptide fused mCherry-UnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
cytoDCIP
Plasmid#193861PurposeEncodes mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.DepositorInsertmCherry-GFP1-10
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1B
Plasmid#198335PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1B
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1A
Plasmid#198334PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRC-N-Venus-V150A-apCC-Di
Plasmid#190706PurposeEncodes a modified version (V150A) of N-Venus followed by the antiparallel coiled-coil apCC-Di.DepositorInsertN-Venus-V150A-apCC-Di
UseTagsExpressionMutationV150APromoteraraBADAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23PI-Sce ESARC D218A
Plasmid#195608PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT928C, T929C, A942CPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23PI-Sce ESARC
Plasmid#195607PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoterDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationC552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…PromoterT7Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
VAMP8
Plasmid#186689PurposeExpresses VAMP8 in E. ColiDepositorInsertVAMP8
UseTags3xFLAG, GST, TEV, and ThrombinExpressionBacterialMutationPromoterT7Available sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-preSufI(IAC)
Plasmid#168516PurposeModified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.DepositorInsertpre-SufI(IAC)
UseTags6xHisC tag and HSV tagExpressionBacterialMutationC17I and C295APromoterT7Available sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Stx3
Plasmid#186685PurposeExpresses Syntaxin 3 in E. ColiDepositorInsertSyntaxin 3
UseTags6 his tagExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
TDRD3:ABC006-C02:C220502
Plasmid#183982PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertTDRD3-anti-TDRD3 FAB (TDRD3 Human)
UseTagsanti-TDRD3 FAB HC: N-terminal STII leader peptid…ExpressionBacterialMutationPromoterAvailable sinceJune 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Mito-CoilE-mEm
Plasmid#181994PurposeCoilE genetically encoded tag on Mito-mEmerald, to be used with CoilR labeled with a reporterDepositorInsertMito-CoilE-mEm
UseTagsmEmeraldExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
TOMM20-CoilE-mCherry
Plasmid#181995PurposeCoilE genetically encoded tag on TOMM2--mCherry, to be used with CoilR labeled with a reporterDepositorInsertTOMM20-CoilE-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PDCD6IP_BRO1
Plasmid#109994PurposeProtein expression and purification of PDCD6IP_BRO1DepositorInsertPDCD6IP_BRO1 (PDCD6IP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LRP5_Ldl-recept-b_2
Plasmid#109807PurposeProtein expression and purification of LRP5_Ldl-recept-b_2DepositorInsertLRP5_Ldl-recept-b_2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBR5_PABP
Plasmid#109877PurposeProtein expression and purification of UBR5_PABPDepositorInsertUBR5_PABP (UBR5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only