Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 12,674 results for: sequence

Showing: 2001 - 2020 of 12674 results

  1. Lyn11-BirA*-HA

    Plasmid
    #214474
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and Lyn11 (Plasma Membrane Localization Se…
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214474-plasmid-map-sequence-id-425600

    Enlarge

  4. pL0_CE-OriT

    Plasmid
    #202150
    Purpose
    Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    Origin of transfer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    OriT sequence mutated to remove sapI sites domest…
    Promoter
    Available since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202150-plasmid-map-sequence-id-405613

    Enlarge

  8. pLY237

    Plasmid
    #184150
    Purpose
    Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 1)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Insert
    s-tracrRNA-R1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD promoter
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184150-plasmid-map-sequence-id-376095

    Enlarge

  9. pLY251

    Plasmid
    #184153
    Purpose
    Reprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Insert
    s-tracrRNA-Ar2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD promoter
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184153-plasmid-map-sequence-id-376090

    Enlarge

  10. pENT-L1-Kozak-HistoneH2B-L2

    Plasmid
    #186357
    Purpose
    Entry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Histone H2B (H2BC11 Human)
    Use
    Expression of a tagged nuclear marker
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186357-plasmid-map-sequence-id-373425

    Enlarge

  18. pBS-V9

    Plasmid
    #173797
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene noctiflora ClpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173797-plasmid-map-sequence-id-342771

    Enlarge

  20. Ash1-5p

    Plasmid
    #169712
    Purpose
    Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed
    Depositor
    Tanja Mittag
    Article
    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323
    Insert
    Ash1 (ASH1 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation including encoding amino acids 420-480…
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169712-plasmid-map-sequence-id-334784

    Enlarge

Showing: 2001 - 2020 of 12674 results