9,002 results

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pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Tags

GST

Expression

Bacterial

Available Since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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MT-cdh1

Plasmid

#11595

Depositor

Marc Kirschner

Article

Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

Insert

cdh1 (FZR1 Human)

Tags

myc

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-GFP_2.0

Plasmid

#11375

Purpose

Retroviral construct for miRNA expression

Depositor

Chang-Zheng Chen

Article

Chen et al Science. 2004 Jan 2. 303(5654):83-6.

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pNatB (pACYCduet-naa20-naa25)

Plasmid

#53613

Purpose

Allows expression of the fission yeast NatB complex - chloramphenicol marker

Depositor

Dan Mulvihill

Article

Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

Inserts

naa20 (naa20 Fission Yeast)
naa25 (naa25 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Abeta(M1-42)

Plasmid

#71875

Purpose

Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-42) (APP Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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spNW15

Plasmid

#173483

Purpose

Expresses spNW15 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW15

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-mitoTPNOX

Plasmid

#87854

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Article

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

mitoTPNOX

Tags

C-terminal FLAG tag with a linker and mitochondri…

Expression

Bacterial

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.LifeAct-GFP.W

Plasmid

#51010

Purpose

Lentivector that expresses LifeAct-GFP from internal human PGK promoter

Depositor

Rusty Lansford

Insert

LifeAct-GFP

Use

Lentiviral

Tags

LifeAct

Expression

Mammalian

Promoter

human PGK

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-hCD19

Plasmid

#127889

Purpose

Expresses hCD19

Depositor

Anjana Rao

Article

Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

Insert

hCD19 (CD19 Human)

Use

Retroviral

Tags

no tags

Mutation

none

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b-RvLEAMshort

Plasmid

#218122

Purpose

Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

Depositor

Ci Ji Lim

Article

Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

Insert

RvLEAMshort

Tags

6xHIS

Expression

Bacterial

Mutation

Truncated form (58-181aa)

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-pA-Hia5

Plasmid

#174372

Purpose

SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

protein A - hia5

Tags

6xHis and Protein A

Expression

Bacterial

Available Since

Sept. 6, 2021

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-Turbo-NES-YFP

Plasmid

#127366

Purpose

Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Tags

GS linker, NES, V5, and YFP

Expression

Plant

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBT375

Plasmid

#125616

Purpose

Expresses evoAPOBEC1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max-NG

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_ORI vector

Plasmid

#99296

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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3xnls-mNeonGreen

Plasmid

#98875

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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LAMP1-RpHLuorin2

Plasmid

#171720

Purpose

Lysosomal expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET_mNG2(1-10)_32aalinker_mNG2(11)

Plasmid

#82611

Purpose

Bacterial expression vector for split mNeonGreen2 screening

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)

Expression

Bacterial

Mutation

split before mutations

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLX307 P2A GFP Luciferase

Plasmid

#193674

Purpose

Constitutive lentiviral expression of Luciferase

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

Luciferase

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Lifeact-7

Plasmid

#54491

Purpose

Localization: Actin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

mCherry-Lifeact

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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murine TCR OTII-2A.pMIG II

Plasmid

#52112

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

P2A (from porcine teschovirus-1)

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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