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We narrowed to 5,413 results for: PEP

Showing: 5051 - 5100 of 5413 results
  1. PGK-HA-PGRN-LAMP1TM

    Plasmid
    #234873
    Purpose
    Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.
    Depositor
    Insert
    Progranulin (GRN Human)
    Use
    Lentiviral
    Tags
    HA tag and LAMP-1 transmembrane domain and cytoso…
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. GRM2-DuET

    Plasmid
    #213301
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    GRM2 (GRM2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. CCR5-DuET

    Plasmid
    #213201
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    CCR5 (CCR5 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. CXCR5-DuET

    Plasmid
    #213222
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    CXCR5 (CXCR5 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. CRHR2-DuET

    Plasmid
    #213216
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    CRHR2 (CRHR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. CCR4-DuET

    Plasmid
    #213200
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    CCR4 (CCR4 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. MSP1-bio

    Plasmid
    #47709
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Insert
    Codon-optimised MSP1 (MSP1 Plasmodium falciparum, Synthetic)
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. EDNRB-DuET

    Plasmid
    #213233
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    EDNRB (EDNRB Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. GHSR-DuET

    Plasmid
    #213251
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    GHSR (GHSR Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. FSHR-DuET

    Plasmid
    #213242
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    FSHR (FSHR Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. CYSLTR1-DuET

    Plasmid
    #213225
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    CYSLTR1 (CYSLTR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. ADORA2A-DuET

    Plasmid
    #213174
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    ADORA2A (ADORA2A Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. CX3C1-DuET

    Plasmid
    #213217
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    CX3C1 (CX3CR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. GPRC5B-DuET

    Plasmid
    #213296
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    GPRC5B (GPRC5B Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  15. A3Ai-Cas9n-UGI-NLS

    Plasmid
    #109425
    Purpose
    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid
    #170185
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.
    Depositor
    Inserts
    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and hU6
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. pCEP4-HLA-c-myc-optiT1R3 ECD-MHC

    Plasmid
    #113961
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonical
    Depositor
    Insert
    T1R3 (TAS1R3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563 fused to a transmembrane tether
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  18. mCerulean-proα1(I)

    Plasmid
    #119842
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  19. pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry

    Plasmid
    #170184
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.
    Depositor
    Inserts
    Sa163 RT
    Retron Sa163 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and hU6
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  20. OPRK1-DuET

    Plasmid
    #213359
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    OPRK1 (OPRK1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177345
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter
    Depositor
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Synapsin
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. pSems leader-ALFA-mXFP-TpoR

    Plasmid
    #222945
    Purpose
    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells
    Depositor
    Insert
    Thrombopoietin receptor (26-635) (MPL Human)
    Use
    Tags
    ALFAtag, Ig k-chain leader sequence, and mXFP
    Expression
    Mammalian
    Mutation
    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…
    Promoter
    CMV
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  23. pMMK ENTR 4 CMV GST mTagBFP

    Plasmid
    #206259
    Purpose
    ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.
    Depositor
    Insert
    GTS mTagBFP
    Use
    Multimate/gateway entr 4
    Tags
    BFGT1 (Golgi localisation peptide)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  24. mApple-proα2(I)-L

    Plasmid
    #120161
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  25. HTR1D-DuET

    Plasmid
    #213318
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    HTR1D (HTR1D Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  26. HTR1B-DuET

    Plasmid
    #213317
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    HTR1B (HTR1B Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  27. pET23a-H6-TEV-cAbl

    Plasmid
    #214234
    Purpose
    Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings
    Depositor
    Insert
    ABL1 Kinase domain (ABL1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  28. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  29. GABBR1-DuET

    Plasmid
    #213247
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    GABBR1 (GABBR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  30. CALCRb-DuET

    Plasmid
    #213193
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    CALCRb (CALCRL Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  31. mApple-proα1(I)

    Plasmid
    #119844
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  32. ADRB2-DuET

    Plasmid
    #213181
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  33. ADRA2A-DuET

    Plasmid
    #213177
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    ADRA2A (ADRA2A Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  34. Cntn1-AP-His

    Plasmid
    #71939
    Purpose
    Expresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Insert
    Cntn1 (Cntn1 Mouse)
    Use
    Tags
    AP-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 5051 - 5100 of 5413 results