We narrowed to 3,050 results for: NAK

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pCS2+ mWnt5a

Plasmid

#102860

Purpose

Expresses mouse Wnt5a in mammalian cells, zebrafish or Xenopus

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Mouse Wnt5a

Use

Zebrafish expression; xenopus expression

Expression

Mammalian

Promoter

CMV; SP6

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSMT3-FBF2

Plasmid

#166972

Purpose

Expresses FBF-2 in E. coli for purification and crystallization studies

Depositor

Traci Hall

Article

Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

Insert

FBF-2 (fbf-2 Nematode)

Tags

His-SUMO

Expression

Bacterial

Mutation

FBF-2 PUM RNA binding domain; amino acids 164-575

Promoter

T7

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast coSHMT2

Plasmid

#106299

Purpose

Expresses SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Codon optimized

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI

Plasmid

#160423

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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NHR1+2-Flag pBacPAK8-3

Plasmid

#17324

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCAG/ATP11B-HA

Plasmid

#209219

Purpose

Mammalian expression of ATP11B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP11B (ATP11B Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-CNL

Plasmid

#65712

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL

Use

Luciferase

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P531A-pBabe-puro

Plasmid

#26091

Depositor

William Kaelin

Article

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA with point mutation P531A

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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CMV-T7-hOsa1

Plasmid

#17986

Depositor

Naoko Tanese

Article

Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

Insert

Osa1 (ARID1A Human)

Tags

T7 epitope

Expression

Mammalian

Mutation

Missing 5' end

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
pLenti CRISPR sgMTHFD2_1

Plasmid

#106313

Purpose

Express Cas9 and sgRNA targeting MTHFD2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2 (MTHFD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304 SLC2A1

Plasmid

#193691

Purpose

Constitutive lentiviral expression of SLC2A1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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TOPO tTR-KRAB-rtTA3-Luc

Plasmid

#68449

Purpose

TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

tTR-KRAB-2A-rtTA3-2A-Luciferase

Use

Luciferase; Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shFXN_1

Plasmid

#102970

Purpose

Suppression of FXN

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shFXN_1 (FXN Human)

Use

Lentiviral and RNAi

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-BLMP-1-pcDNA3.1-

Plasmid

#52512

Purpose

expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blmp-1 (blmp-1 Nematode)

Tags

FLAG-HA

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shFXN_2

Plasmid

#102971

Purpose

Suppression of FXN

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shFXN_2 (FXN Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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R26TV LSL-TRL

Plasmid

#68476

Purpose

Rosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassette

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

tTR-KRAB-2A-rtTA3-2A-Luciferase

Use

Cre/Lox, Luciferase, and Mouse Targeting

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTorPE-GEM-GECO1

Plasmid

#32463

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO1

Use

Lab constructed

Tags

6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLVXT-mTcf3

Plasmid

#121935

Purpose

doxycycline-inducible mouse Tcf3

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Transcription Factor 3 (Tcf3 Mouse)

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-Gsk3b(new)

Plasmid

#122341

Purpose

Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Gsk3b

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRBP-L1+D1+L2-myc/His

Plasmid

#180599

Purpose

Expressing various parts of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

Insert

TRBP (TARBP2 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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