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We narrowed to 70,504 results for: LAS

Showing: 3061 - 3090 of 70504 results

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hP14Gal4

Plasmid

#84364

Purpose

Gal4-PRDM14 funsion plasmid for luciferase recruitment assay

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Use

Tags

Gal4 and HA tag

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CMV/TO

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCR4 hNFATc1-EE

Plasmid

#17857

Depositor

Jerry Crabtree

Article

Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

Insert

NFATc1 (NFATC1 Human)

Use

Tags

EE

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Use

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat FL

Plasmid

#32449

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S3L, G96R, and P108L

Promoter

SV40

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only
pEN_TGmiR_Luc-A

Plasmid

#25765

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF1b

Plasmid

#67494

Purpose

Expression of shRNA targeting human ARF1b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF1 shRNA (ARF1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEN_TRmiRc2

Plasmid

#25750

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

dsRed2

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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Pdar

Plasmid

#24786

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Pdar

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2011

Availability

Academic Institutions and Nonprofits only
GST-RB (829-928)

Plasmid

#31498

Depositor

Julien Sage

Article

Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

Insert

RB (RB1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 829-928

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
GST-RB (736-840)

Plasmid

#31497

Depositor

Julien Sage

Article

Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

Insert

RB (RB1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 736-840

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pEN_hU6miR-Luc-A

Plasmid

#25756

Purpose

Entry vector with human U6 promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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TR- ATG-HP-GFP

Plasmid

#80592

Purpose

Expresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Inserted 30 nt hairpin which is targeted by Csy4 …

Promoter

CBA

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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GST-RB (561-673)

Plasmid

#31495

Depositor

Julien Sage

Article

Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

Insert

RB (RB1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 561-673

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
Prb

Plasmid

#24785

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
Pdar(Asn34Asp)

Plasmid

#24834

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Pdar Asn34Asp

Use

Tags

HA and Myc

Expression

Mammalian

Mutation

Asn34Asp

Promoter

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only
Prb(Asp83Asn)

Plasmid

#24833

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb(Asp83Asn)

Use

Tags

Avi and His

Expression

Bacterial

Mutation

Asp83Asn

Promoter

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
GST-RB (654-752)

Plasmid

#31496

Depositor

Julien Sage

Article

Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

Insert

RB (RB1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 654-752

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
mCherry-FKBP-TPD54

Plasmid

#172451

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

Insert

TPD54 (TPD52L2 Human)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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TR-5'UTR-HP-GFP

Plasmid

#80594

Purpose

Expresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Inserted 30 nt hairpin which is targeted by Csy4 …

Promoter

CBA

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMK1224

Plasmid

#84221

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFP

Depositor

Jonathan Weissman, Martin Kampmann

Article

Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Tags

Expression

Mutation

no stop codon

Promoter

none

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF3b

Plasmid

#67496

Purpose

Expression of shRNA targeting human ARF3b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF3 shRNA (ARF3 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat K193A

Plasmid

#32450

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K193A (S3V, G96R, and P108L also present)

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)MBII-85-3'ss mut

Plasmid

#67644

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Use

Tags

Expression

Mammalian

Mutation

3' splice site muattion

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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PdarC1

Plasmid

#24835

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PdarC1

Use

Tags

HA and Myc

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K651R

Plasmid

#84528

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Tags

Expression

Mutation

K651R

Promoter

none

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PrbC10

Plasmid

#24788

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Use

Tags

His

Expression

Bacterial

Mutation

Ile75Val, Asp83Asn, Leu122Pro, and Glu135Lys

Promoter

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
PBB-79

Plasmid

#8813

Depositor

Cynthia Wolberger

Article

Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

Insert

GABPb1 (1-157) (Gabpb1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat D311A

Plasmid

#32451

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D311A (S3V, G96R, and P108L also present)

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only