We narrowed to 2,496 results for: 1186

1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. ELK4_pet28a

   Plasmid

   #131648

   Purpose

   Bacterial expression of ETS gene ELK4

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELK4 (ELK4 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ELF4_pet28a

   Plasmid

   #131661

   Purpose

   Bacterial expression of ETS gene ELF4

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELF4 (ELF4 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR P2R-P3-ECFP

   Plasmid

   #48353

   Purpose

   Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   ECFP
   

   Use

   Gateway Cloning

   Mutation

   Contains a stop codon.

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIMVAd39T

   Plasmid

   #98303

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

   Expression

   Yeast

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-Flag-dSpCas9

   Plasmid

   #92113

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR P4-P1R-mKate2

   Plasmid

   #48344

   Purpose

   Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   mKate2
   

   Use

   Gateway Cloning

   Mutation

   It contains a Kozak sequence but no stop codon.

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR P4-P1R-EYFP

   Plasmid

   #48350

   Purpose

   Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EYFP
   

   Use

   Gateway Cloning

   Mutation

   Contains a Kozak sequence. Does not contain a sto…

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 RL3Sc

   Plasmid

   #109365

   Purpose

   Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
   

   Tags

   1D4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ELF5_pet28a

   Plasmid

   #131659

   Purpose

   Bacterial expression of ETS gene ELF5

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELF5 (ELF5 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. promoter J23110 (upstream of RBS, mRFP1)

    Plasmid

    #117852

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    medium-high promoter, RBS, mRFP1
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA

    Plasmid

    #97012

    Purpose

    Rosa26 targeting vector for intersectional Cre and FLP responsive cassette

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA

    Plasmid

    #97010

    Purpose

    Rosa26 targeting vector for FLP-responsive cassette

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC240

    Plasmid

    #118895

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and mCherry, clea…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDECKO_mCherry_CD19.4

    Plasmid

    #140101

    Purpose

    CRISPR-Cas9 Knock down

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBI101-pGC1(D1)-GUS

    Plasmid

    #49513

    Purpose

    Expresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plant

    Depositor

    Julian Schroeder

    Article

    Yang et al Plant Methods. 2008 Feb 19;4:6. doi: 10.

    Insert

    Promoter of GC1(D1) (AT1G22690 Mustard Weed)
    

    Use

    Plant expression

    Tags

    beta-glucuronidase

    Promoter

    GC1 (At1g22690), 1141 bp upstream of the transcri…

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pDONR P2R-P3-V5-6xHis

    Plasmid

    #48347

    Purpose

    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    V5 and 6xHis epitope tags cassette
    

    Use

    Gateway Cloning

    Mutation

    Contains a stop codon at the end of the epitope t…

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. p1.1-eGFP

    Plasmid

    #162738

    Purpose

    Fluorescent reporter for CHO cells expression studies

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Insert

    enhanced green fluorescent protein
    

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEM-HE-Venus-bPAC(F198Y)

    Plasmid

    #165487

    Purpose

    Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    Venus-bPAC(F198Y)-myc
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Myc and Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC120

    Plasmid

    #118892

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and mGFP, HIS6, c…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQTEV-FBXO31

    Plasmid

    #34815

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    F-box protein 31 (FBXO31 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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