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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 30,934 results for: LIS;

Showing: 2041 - 2060 of 30934 results

  1. pSBtet-RH-β+ε

    Plasmid
    #247178
    Purpose
    For generating Sleeping Beauty-based stable cell line expressing human muscle acetylcholine receptor beta1 and epsilon subunits. Expresses RFP; hygromycin selection.
    Depositor
    Ryan Hibbs
    Article
    Li et al Cell. 2025 May 1;188(9):2390-2406.e20. d
    Insert
    Human muscle acetylcholine receptor beta and epsilon subunits
    Tags
    3xFLAG tag
    Expression
    Mammalian
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    247178-plasmid-map-sequence-id-491383

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  2. pSBtet-GP-α+δ

    Plasmid
    #247177
    Purpose
    For generating Sleeping Beauty-based stable cell line expressing human muscle acetylcholine receptor alpha1 and delta subunits. Expresses GFP; puromycin selection.
    Depositor
    Ryan Hibbs
    Article
    Li et al Cell. 2025 May 1;188(9):2390-2406.e20. d
    Insert
    Human muscle acetylcholine receptor alpha1 and delta subunits
    Tags
    Twin strep tag
    Expression
    Mammalian
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    247177-plasmid-map-sequence-id-491317

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  18. pXeDNA3.1-LacZ

    Plasmid
    #242746
    Purpose
    pXeDNA3-LacZ TA-cloning vector for dual expression: CMV enhancer/promoter in mammalian cells and Xenopus β-globin 5'/3'UTRs in oocytes; BGHpA, AmpR, NeoR, lacZα for blue-white screening.
    Depositor
    Luis Yanez-Guerra
    Type
    Empty backbone
    Use
    Xenopus oocyte expression
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    242746-plasmid-map-sequence-id-487866

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  19. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Mikko Taipale, Benjamin Blencowe
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  20. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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Showing: 2041 - 2060 of 30934 results