We narrowed to 58,540 results for: Ran

1. KLHL31 pgk (1088)

   Plasmid

   #62031

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   KLHL31 (Klhl31 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA5-Flag-Med25

   Plasmid

   #64771

   Purpose

   Mammalian protein expression of flag-tagged Med25

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   Med25 (MED25 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

3. mEos3.2FPP

   Plasmid

   #203758

   Purpose

   Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   HRas c-term (HRAS Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. hU6-pegRNA-EF-1α-Cre Template

   Plasmid

   #196036

   Purpose

   The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

   Depositor

   Tyler Jacks

   Article

   Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hU6 and EF-1-alpha

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. IL2R/hE-cadherin-cytotail

   Plasmid

   #45773

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)
   

   Expression

   Mammalian

   Mutation

   Interleukin-2 receptor α subunit extracellular an…

   Promoter

   CMV

   Available Since

   June 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. TAPBPL pmRFP-N2 (721)

   Plasmid

   #62000

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   TAPBPL (TAPBPL Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1_Tau Authentic Alu 10-12V337M

   Plasmid

   #194169

   Purpose

   Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 V337M
   

   Tags

   3X flag

   Expression

   Mammalian

   Mutation

   Changed Valine 337 to Methinonine

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-Med25-VBD-6His

   Plasmid

   #64773

   Purpose

   Bacterial expression of His-tagged Med25-VBD

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   Med25-VBD (MED25 Human)
   

   Expression

   Bacterial

   Mutation

   insert is 394-543 only

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

9. TMEM216-mCherry

   Plasmid

   #41633

   Depositor

   Joseph Gleeson

   Article

   Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

   Insert

   Transmembrane Protein 216 (TMEM216 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pAF164

    Plasmid

    #135928

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mOrange fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    TNFRSF9 fused to mOrange
    

    Use

    Transposon

    Tags

    mOrange

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TMEM138-mCherry

    Plasmid

    #41630

    Depositor

    Joseph Gleeson

    Article

    Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

    Insert

    Transmembrane Protein 138 (TMEM138 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pET41s-GST-ATF6a-AD

    Plasmid

    #64774

    Purpose

    bacterial expression of GST-tagged ATF6 (1-150)

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    ATF6a-AD (1-150) (ATF6 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-term deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

    Plasmid

    #172195

    Purpose

    AAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

    Depositor

    Garrett Kaas

    Article

    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

    Insert

    HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
    

    Use

    AAV and Mouse Targeting; Tale

    Tags

    HA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    transcription regulatory domain removed

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-Flag-Med25-VWA

    Plasmid

    #64772

    Purpose

    Mammalian expression of Flag-tagged Med25-VWA

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VWA (MED25 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA TMEM 138-flag

    Plasmid

    #41628

    Depositor

    Joseph Gleeson

    Article

    Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

    Insert

    Transmembrane Protein 138 (TMEM138 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pBiFC-SS-VN155(I152L)-IRE1-dLD

    Plasmid

    #217752

    Purpose

    Expresses the VN fragment of BiFC-IRE1-dLD

    Depositor

    Christina Chan

    Article

    Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

    Insert

    Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)
    

    Tags

    Venus (1-154, I152L)

    Expression

    Mammalian

    Mutation

    deleted amino acids 29-408

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-CLIP-CD28(CD45TM)

    Plasmid

    #223629

    Purpose

    Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Mutation

    CD28 transmembrane domain replaced by CD45 transm…

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-Spy-CD28(CD45TM)

    Plasmid

    #223624

    Purpose

    Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Spytag003

    Mutation

    CD28 transmembrane domain replaced by CD45 transm…

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLC045 - pL0_CrGLK (CDS1ns)

    Plasmid

    #203904

    Purpose

    MoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    CrGLK (without a stop codon)
    

    Expression

    Plant

    Mutation

    Silent mutation of BpiI or BsaI cut site for MoCl…

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUN1213 - pL0_p16OMT (pro + 5U)

    Plasmid

    #203890

    Purpose

    16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    16OMT promoter and 5'UTR
    

    Expression

    Plant

    Mutation

    Mutation of BpiI and/or BsaI cut sites for MoClo …

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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