We narrowed to 5,822 results for: pcas

1. Osa-pCAMBIA-1301-STTM529

   Plasmid

   #84225

   Purpose

   Disturb the expression of miR529 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR529
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

2. Osa-pCAMBIA-1301-STTM530-3p

   Plasmid

   #84226

   Purpose

   Disturb the expression of miR530-3p in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR530-3p
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gma-pCAMBIA-1301-STTM396

   Plasmid

   #84081

   Purpose

   Disturb the expression of miR396 in soybean

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR396
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gma-pCAMBIA-1301-STTM408

   Plasmid

   #84082

   Purpose

   Disturb the expression of miR408 in soybean

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR408
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

5. Osa-pCAMBIA-1301-STTM397

   Plasmid

   #84207

   Purpose

   Disturb the expression of miR397 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR397
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459V2.0-eSpCas9(1.1)+K855A

   Plasmid

   #108298

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459V2.0-eSpCas9(1.1)+K810A

   Plasmid

   #108297

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9 (BB)-2A-GFP gamma-tubulin sg

   Plasmid

   #104437

   Purpose

   Knockdown the expression of gamma-tubulin in human cells

   Depositor

   Maria Alvarado-Kristensson

   Article

   Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

   Insert

   gamma-tubulin sgRNA
   

   Tags

   The gamma-tubulin sgRNA is coexpressed with 3xFLA…

   Expression

   Mammalian

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW706_pCAG-PV2-lox2272-GFP

   Plasmid

   #87570

   Purpose

   PV2 for 2-input, 2-output circuit library

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCASCADE-gltA1-gltA2-GapAP1

    Plasmid

    #87150

    Purpose

    gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gltA1-gltA2-gapAP1 guide array
    

    Expression

    Bacterial

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-Flag-HFSpCas9-H840A

    Plasmid

    #80455

    Purpose

    high fidelity SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    HFSpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. -161+80pCalb2/mutE2F(-69)-like

    Plasmid

    #66743

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, mutant)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    GCG (-69,-68,-67) to ATT

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-CD4-YEML-myc

    Plasmid

    #58538

    Purpose

    Expresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    CD4 (CD4 Human)
    

    Tags

    IFITM3 endocytosis Tyrosine-Glutamate-Methionine-…

    Expression

    Mammalian

    Mutation

    Added C-terminal Tyrosine-Glutamate-Methionine-Le…

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-RFP-miR-E2-4int

    Plasmid

    #19824

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Insert

    miRNA
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-pCALM1-EYFP-WPRE-bGHpA

    Plasmid

    #213971

    Purpose

    AAV expression of EYFP from pCALM1 promoter

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    pCALM1
    

    Use

    AAV

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Expression

    Mammalian

    Mutation

    H840A

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV pCAG-FLEX-EGFP-WPRE

    Plasmid

    #51502

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

    Plasmid

    #103849

    Purpose

    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    CMV

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid

    #181745

    Purpose

    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    human/zebrafish codon optimized SpCas9
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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