We narrowed to 4,145 results for: pes

1. pCas12(PI)

   Plasmid

   #186448

   Purpose

   Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12a (PI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K613A, K617A

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas12b

   Plasmid

   #186449

   Purpose

   Encodes Cas12b from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12b
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas12b(PI)

   Plasmid

   #186451

   Purpose

   Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12b (PI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   R122A, G143P

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWPT-mEGFP

   Plasmid

   #190606

   Purpose

   Obtained by creating a deletion inside the mCherry coding sequence in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235)

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a deletion was created in mCherry CDS impairing i…

   Promoter

   EIF1-short

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

   Plasmid

   #169597

   Purpose

   Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9 - VP64 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTL464

   Plasmid

   #69881

   Purpose

   a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

   Depositor

   Tobias Langenhan, Robert Kittel

   Article

   Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

   Insert

   CIRL (Cirl Fly)
   

   Use

   Phic31-integration vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FluoSTEP-AKAR (C/Y)

   Plasmid

   #181862

   Purpose

   FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AKAR (C-Y)
   

   Use

   Tags

   CFP(1-10) and cpVenus(E127)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-MNTS-1-p75

   Plasmid

   #182945

   Purpose

   To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTR

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.

   Insert

   MNTS-1-p75 (NTF3, NT3 Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter and enhancer

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-MNTS-1

   Plasmid

   #182944

   Purpose

   To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTR

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.

   Insert

   MNTS-1 (NTF3 Homo sapiens, Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   This is the Human NT-3 with mutations on the matu…

   Promoter

   CMV promoter and enhancer

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOME0233M

    Plasmid

    #178415

    Purpose

    Reverse compl seq of pOME0134 flanked by Kpn and EcoRI for insertion into pcDNA3.1-

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    Reverse complement of pOME0134 upstream MIR-SYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOME0232M

    Plasmid

    #178414

    Purpose

    REVERSE complement seqeunce KSHV Downstream Mir-SYN RNA IN PCDNA3.1-

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    KSHV Reverse complement of downstream MIR-SYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p23-NES-RanCas13b-msfGFP-NES-Flag

    Plasmid

    #165073

    Purpose

    overexpression of RanCas13b in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    RanCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUE1-TSP-ORF68 K174A/R179A/K182A

    Plasmid

    #162654

    Purpose

    Expresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K174, R179, and K182 mutated to alani…

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO-ORF24 1-201-2xStrep

    Plasmid

    #138442

    Purpose

    Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-201

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA4/TO-ORF24 1-226-2xStrep

    Plasmid

    #138443

    Purpose

    Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-226

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4/TO-BcRF1 1-168-2xStrep

    Plasmid

    #138447

    Purpose

    Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1 (BcRF1 EBV (HHV-4))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-168

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/TO-BcRF1 1-178-2xStrep

    Plasmid

    #138448

    Purpose

    Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1 (BcRF1 EBV (HHV-4))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-178

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4/TO-BcRF1 1-203-2xStrep

    Plasmid

    #138449

    Purpose

    Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1 (BcRF1 EBV (HHV-4))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-203

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO-UL87 1-238-2xStrep

    Plasmid

    #138450

    Purpose

    Expresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    UL87 (UL87 HCMV (HHV-5))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-238

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-UL87 1-248-2xStrep

    Plasmid

    #138451

    Purpose

    Expresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    UL87 (UL87 HCMV (HHV-5))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Amino acids 1-248

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge