162,717 results

1. pMG024 MBP-GSK3β-HA-His, GST_λPPase

   Plasmid

   #196182

   Purpose

   Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Inserts

   GSK3B (GSK3B Human)
   λPPase
   

   Use

   Tags

   GST, HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAK_DR30_CasRx_mCherry

   Plasmid

   #134844

   Purpose

   All in one overexpression of CasRx and guideRNA

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   T2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG.Ruby2sm-Flag.WPRE.SV40

   Plasmid

   #98925

   Purpose

   AAV expression of Ruby2 spaghetti monster FLAG construct

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   Ruby2-smFP-Flag
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ER-RpHLuorin2

   Plasmid

   #171717

   Purpose

   Luminal ER expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 targeted to ER
   

   Use

   Tags

   KDEL ER-retention signal and Signal sequence of h…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGW1-Myc-DJ1-WT

   Plasmid

   #29347

   Depositor

   Mark Cookson

   Article

   Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

   Insert

   DJ1 (PARK7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO_Vim-SNAP-tag2

   Plasmid

   #226518

   Purpose

   Mamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   Vimentin-SNAP-tag2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 457 p3E-2A-EGFPpA

   Plasmid

   #195967

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-EGFP
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. DsRed-Rab5 DN

   Plasmid

   #13051

   Depositor

   Richard Pagano

   Article

   Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

   Insert

   RAB5A (Canis familiaris) (RAB5A Canis familiaris)
   

   Use

   Tags

   DsRed2 and HA

   Expression

   Mammalian

   Mutation

   asparagine 3 changed to serine (neutral); aspara…

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1A-CD19.28H.28TM.28.BB.3z

   Plasmid

   #200680

   Purpose

   Modular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD28HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3258 pCMV hUBC9 wt HA

    Plasmid

    #14438

    Depositor

    Peter Howley

    Article

    Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge