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pFA6a-BIO-hphMX4

Plasmid

#26880

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

BIO-tag

Use

Pcr-tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pILGFP5V1

Plasmid

#165058

Purpose

Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1- T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3

Plasmid

#112658

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

sfGFP-Glo3

Tags

sfGFP

Expression

Yeast

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pXP212

Plasmid

#26828

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP5

Plasmid

#87592

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp5

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-BIO-TRP1

Plasmid

#26881

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

BIO-tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pXP116

Plasmid

#26813

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pXP120

Plasmid

#26826

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymYPET-T2A-mTurquoise2

Plasmid

#118444

Purpose

Produces equimolar expression of ymYPET and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET-T2A-mTurquoise2

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 Y292C

Plasmid

#87291

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 Y292C

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

Y292C

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pK3F-PYK1

Plasmid

#85771

Purpose

N-ICE plasmid pK3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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YIplac204-T|C-iGFP-RCY1

Plasmid

#105281

Purpose

Overexpresses iGFP-RCY1 from the TPI1 promoter. Integrating at TRP1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Rcy1 (RCY1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Promoter

TPI1

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGRE NPD-GFP

Plasmid

#1197

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

N-terminus of Sup35 fused to GFP (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

fused N-terminal Sup35 (~1-253aa) to GFP

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pCZ45ai

Plasmid

#16469

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

p53 binding site (TP53 Human)

Expression

Yeast

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pXP312

Plasmid

#26834

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinADE E171Q GFP

Plasmid

#32128

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303, E171Q

Available Since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
pPS934

Plasmid

#8855

Depositor

Pamela Silver

Article

Kahana et al Mol Biol Cell. 1998 Jul . 9(7):1741-56.

Insert

NUF2-sGFP (NUF2 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pFA6a-RGS18HIS-TRP1

Plasmid

#26884

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

18xHis tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinADE HDEL

Plasmid

#32118

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

HDEL and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303

Available Since

Oct. 12, 2011

Availability

Academic Institutions and Nonprofits only
pPS1597

Plasmid

#8920

Depositor

Pamela Silver

Article

Valentini et al Genetics. 2002 Feb . 160(2):393-405.

Insert

TIF51A (HYP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pXP112

Plasmid

#26805

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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p316CUPSup35GFPSG

Plasmid

#1224

Depositor

Susan Lindquist

Article

Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

Insert

SUP35 (SUP35 Budding Yeast)

Tags

GFPSG S65T V164A

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pEB0_13_PGK1p-QF-ACT1t

Plasmid

#236216

Purpose

Plasmid encoding PGK1p-QF2-ACT1t as a combined Type 234 part to be used in the Dueber Yeast MoClo Toolkit.

Depositor

Yvonne Nygård

Article

Blick et al Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf0

Insert

QF, ACT1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PGK1p

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pRS426-SNR52-crRNA

Plasmid

#251984

Purpose

Expression of crRNA (version 3) for editing, used together with tracrRNA Plasmid #251985. This plasmid is also called crRNA in the paper.

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

crRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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HRPEadh-pAG303

Plasmid

#249735

Purpose

Derived from pAG303GPD-ccdb (#14134); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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HRPEadh-pAG305

Plasmid

#249734

Purpose

Derived from pAG305GPD-ccdb (#14138); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAG304-ccdbNLUC

Plasmid

#249736

Purpose

Derived from pAG304GPD-ccdb (#14136); GPD removed and NLUC cds inserted downstream gateway cassette. Useful for cloning promoter-NLUC fusions in Saccharomyces cerevisiae

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pNH605-Padh-AtAFB2

Plasmid

#247611

Purpose

Expression of AtAFB2

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

AtAFB2

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pNH605-Padh-OsTIR1

Plasmid

#247609

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRS405-Padh-AtAFB2

Plasmid

#247600

Purpose

Expression of AtAFB2

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

AtAFB2

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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