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pCT-CMV-UBE3BΔHECT-copGFP-Puro
Plasmid#107343PurposeExpresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.DepositorInsertUBE3B(ΔHECT)-copGFP (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationdeletion of HECT domain (757-end)PromoterCMVAvailable sinceJune 26, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
HOXA9 (murine) FLAG pRC/CMV
Plasmid#8514DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionMammalianMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Actc1
Plasmid#99691PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Mouse, Synthetic)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
p7055 pHAGE-P-CMVt-N-HA-GAW-COPZ1
Plasmid#100145PurposeExpresses COPZ1DepositorInsertCOPZ1 (COPZ1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
ptdTomato-N1-CMVL-ikk1FL(C179A)-tdTomato
Plasmid#105644PurposeZebrafish Inhibitor of kappa B kinase alpha with mutation at position 179 from cysteine to alanineDepositorInsertCMVL-ikk1FL(C179A)-tdTomato (chuk Zebrafish)
UseTagstdTomatoExpressionMammalianMutationCMV:ikk1FL(C178A)-tdTomatoPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)
Plasmid#115140PurposeCMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLSDepositorInserthuman codon optimized AcrVA3 anti-CRISPR protein
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)
Plasmid#115138PurposeCMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLSDepositorInserthuman codon optimized AcrVA2 anti-CRISPR protein
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag p38 alpha(agf)
Plasmid#20787DepositorInsertpCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationreplaced Thr180 and Tyr182 with Ala and Phe, resp…PromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
Construct 7 - CMVp-Cas9-3xNLS-HSVpA
Plasmid#81247PurposeExpresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expressionDepositorInsertCas9-3xNLS
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVH227-Tier1-OTetO7-PCMVmin2-SEAP
Plasmid#169563PurposeTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).DepositorInserttetracycline-controlled SEAP production
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVminAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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p3xFLAG-CMV TIP60 212-364
Plasmid#78795PurposeTo overexpress TIP60 212-364 in Mammalian CellsDepositorInsertTIP60 (KAT5 Human)
UseTags3xFLAGExpressionMammalianMutation212-364, G214APromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Neurog2
Plasmid#99695PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceSept. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D-CD4-FLAG
Plasmid#51601Purposeexpresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4DepositorInsertSema4D/CD4-FLAG (Sema4d Human, Mouse)
UseTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV2-Brd4 FEE-AAA
Plasmid#21942DepositorInsertBRD4 (BRD4 Human)
UseTagsFlagExpressionMammalianMutationaa 1209-1362 only, FEE-AAAPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Drosha (NLS-P426)
Plasmid#171538PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
UseTagsExpressionMammalianMutationan SV40 NLS sequence (PKKKRKVGI) insertion into t…PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S127/384A
Plasmid#33076DepositorInsertYAP (YAP1 Human)
UseTagsFlagExpressionMammalianMutationS127A, S384APromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6
Plasmid#159913PurposeMutagenesis of Kcnj6DepositorInsertKcnj6 (Kcnj6 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRb delta CDK + S230
Plasmid#58907Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S230 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + T252
Plasmid#58910Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with T252 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)
Plasmid#134334PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA17_Sga
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)
Plasmid#134335PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA18_Sma
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)
Plasmid#134336PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA19_Ssim
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)
Plasmid#134338PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA18_Sga
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)
Plasmid#134339PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA19_Spse
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCMV superYFP-AURKA K162M-mTurq2
Plasmid#157773PurposeExpression of AuroraA kinase-dead biosensor under CMV promoterDepositorInsertAURKA (AURKA Human)
UseTagsmTurquoise2 and superYFPExpressionMammalianMutationAURKA K162M is a kinase-dead version of AURKAPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S127/387A
Plasmid#33079DepositorInsertYAP (YAP1 Human)
UseTagsFlagExpressionMammalianMutationS127A, S387APromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-RNF8-N-terminus(1-111)
Plasmid#64674PurposeExpresses only N terminus of RNF8, amino acids 1 to 111DepositorInsertRing finger protein 8 (RNF8 Human)
UseTagsFLAGExpressionMammalianMutationOnly N terminus amino acids 1-111PromoterCMVAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-CMV-p85a 3'UTR mutant
Plasmid#51393DepositorInsertp85alpha 3'UTR (PIK3R1 Human)
UseLuciferaseTagsLuciferaseExpressionMutationmiR-29 binding site mutantPromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shRen-Blast
Plasmid#135684PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Blast
Plasmid#135686PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-R1868*-3xFLAG
Plasmid#130679PurposeExpresses human SMCHD1 R1868* with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 R1868* (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationR1868_V2005delinsXPromoterCMVAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-GABARAPL1 G116A
Plasmid#123118PurposeExpresses EGFP-GABARAPL1 G116A in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseTagsEGFPExpressionMammalianMutationGlycine 116 to AlaninePromoterCMVAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)
Plasmid#119316Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 481-852PromoterCMVAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-XPLN aa452-526
Plasmid#73381PurposeExpresses amino acids 452-526 of XPLN (Arhgef3) proteinDepositorInsertarhgef3 (ARHGEF3 Human)
UseTagsMyc (QKLISEEDL)ExpressionMammalianMutationExpresses amino acids 452-526 of XPLN (arhgef3) p…PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-XPLN aa304-466 (PH)
Plasmid#73380PurposeExpresses amino acids 304-466 of XPLN (Arhgef3) proteinDepositorInsertarhgef3 (ARHGEF3 Human)
UseTagsMyc (QKLISEEDL)ExpressionMammalianMutationExpresses amino acids 304-466 of XPLN (arhgef3) p…PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-XPLN aa107-311
Plasmid#73379PurposeExpresses amino acids 107-317 of XPLN (Arhgef3) proteinDepositorInsertarhgef3 (ARHGEF3 Human)
UseTagsMyc (QKLISEEDL)ExpressionMammalianMutationExpresses amino acids 107-317 of XPLN (arhgef3) p…PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only
