We narrowed to 2,496 results for: 1186
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Plasmid#1186DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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HeFm2SpCas9
Plasmid#92111PurposeExpression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)DepositorInsert“Highly enhanced Fidelity” mut2 SpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, K848A, Q926A, R1060APromoterCbhAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKS7107
Plasmid#89051PurposeExpresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9DepositorInsertsNm crRNA
Nm tracrRNA
hNmCas9
UseCRISPRTagsHA tag, NLS, and SV40 NLSPromoterEF1a and human U6Available SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) dRhoA V14 (CT#846)
Plasmid#13949DepositorAvailable SinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMiR-E6AP-3'UTR
Plasmid#53694PurposeLuciferase reporter assay for microRNA binding on E6AP 3'UTRDepositorInsertUBE3A 3'UTR (UBE3A Human)
UseLuciferaseAvailable SinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
p1.2-Zeo-eGFP
Plasmid#162743PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MAPRE1
Plasmid#31589DepositorInsertmicrotubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-2xAM_hIRF-1
Plasmid#92221PurposeLentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoterDepositorInsertsSp-dCas9-2xAM tag
gRNA targeting human IRF-1 promoter
UseCRISPR and LentiviralTags2xAM tagExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterCBh and U6Available SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-GTF2F2
Plasmid#31323DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) dRhoA N19 (CT#847)
Plasmid#14002DepositorAvailable SinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
Pk_CyRPA-Cd4-bio-His
Plasmid#126841PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertCyRPA (PKH_052740 Plasmodium knowlesi)
TagsratCd4(d3+4)-bio-6HisExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-KCNIP2
Plasmid#34799DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOCC94
Plasmid#118912Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and eGFP-HIS6ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-AK1
Plasmid#31290DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
p35S::IRE1a
Plasmid#135232Purposeconstitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)DepositorAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC254
Plasmid#118889Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease; mCherry, cl…ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPTXcGMPRELUC
Plasmid#68503Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
TagsluciferaseExpressionBacterial and PlantMutation3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…PromoterOPTX promoter with cGMPREAvailable SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Am
Plasmid#109364PurposeHuman rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tagDepositorInsertRod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Branchiostoma belcheri, Human)
Tags1D4ExpressionMammalianPromoterCMVAvailable SinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_RNeoStop_WPRE_bgh_pA
Plasmid#97011PurposeRosa26 targeting vector for Dre-responsive cassetteDepositorTypeEmpty backboneUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-COASY
Plasmid#34816DepositorAvailable SinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only