We narrowed to 5,803 results for: SUP

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pGEMT-blastR-p2a-v5-dL5

Plasmid

#161980

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

blastR-p2a-v5-dL5-GSlinker

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-v5-dL5-p2a-blastR

Plasmid

#161981

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GSlinker-V5-dL5-P2A-blastR

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-HiGlpG- S116A, H196A

Plasmid

#158539

Purpose

Bacterial expression of rhomboid protease catalytic mutant HiGlpG-S116A,H196A

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

HiGlpG

Tags

GST tag

Expression

Bacterial

Mutation

S116A;H169A

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-delN-EcGlpG

Plasmid

#158537

Purpose

Bacterial expression of rhomboid protease EcGlpG without N terminus

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

delta N-terminus EcGlpG

Tags

GST tag

Expression

Bacterial

Mutation

N-terminal deletion (1-86)

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pNIC28-BpWcbI

Plasmid

#125591

Purpose

Overexpression plasmid for production of WcbI from Burkholderia pseudomallei

Depositor

Nicholas Harmer

Article

Vivoli et al IUCrJ. 2013 Oct 18;1(Pt 1):28-38. doi: 1

Insert

BpWcbI (wcbI Burkholderia pseudomallei)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCASCADE-fabI-gltA1-gltA2-udhA-zwf-gapAP1

Plasmid

#87149

Purpose

fabI-gltA1-gltA2-udhA-zwf-gapAP1 targeting guide RNA E. coli, Low Phosphate Induction

Depositor

Michael Lynch

Insert

fabI-gltA1-gltA2-udhA-zwf-gapAP1 guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBsaFa

Plasmid

#85104

Purpose

FAD domain of P450 BM3 variant (C773S/C810S)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

FADdomain of P450 BM3

Use

Cloning vector

Mutation

C773S/C810S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pBsaPro5X

Plasmid

#85100

Purpose

Putidaredoxin fused to Pro5

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Putidaredoxin

Use

Cloning vector

Mutation

changed Cysteine 73 to Serine and Cysteine 85 to …

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pHPro51

Plasmid

#85097

Purpose

1st Pro5

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Gly-Gly-Gly-Gly-Ser-Pro-Pro-Pro-Pro-Pro

Use

Cloning vector

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSEVA12TEC-B

Plasmid

#58307

Purpose

GeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

Genomic cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pME18S-sc1686

Plasmid

#52390

Purpose

Aedes aegypti NPY-like receptor super contig 1.686 (NPYLR6) full-length clone in mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

NPYLR6

Expression

Mammalian

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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pME18S-sc1156

Plasmid

#52389

Purpose

Aedes aegypti NPY-like receptor super contig 1.156 (NPYLR5) full-length clone in mammalian expression vector

Depositor

Leslie Vosshall

Article

Liesch et al PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2

Insert

NPYLR5

Expression

Mammalian

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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tout velou (ttv)

Plasmid

#37401

Depositor

Norbert Perrimon

Article

Bellaiche et al Nature. 1998 Jul 2;394(6688):85-8.

Insert

tout-velu (ttv Fly)

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pGEX2T E7 del PTLHE

Plasmid

#13637

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 del PTLHE (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

del aa 6-10

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pMM95

Plasmid

#176615

Purpose

Extracellular domain truncated. Used to test SIRPA exclusion from the phagocytic synpase.

Depositor

Meghan Morrissey, Ron Vale

Article

Morrissey et al Immunity. 2020 Aug 18;53(2):290-302.e6.

Insert

FRBext-GFPint

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Availability

Academic Institutions and Nonprofits only

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pMM98

Plasmid

#176617

Purpose

Contains 3 fibcon repeats to observe SIRPA exclusion from the phagocytic synapse

Depositor

Meghan Morrissey, Ron Vale

Article

Morrissey et al Immunity. 2020 Aug 18;53(2):290-302.e6.

Insert

FRB-FIB3ext-GFPint

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Availability

Academic Institutions and Nonprofits only

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pLenti-puro

Plasmid

#39481

Purpose

Tetracycline inducible 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

V5 and 6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pHD066

Plasmid

#84032

Purpose

Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafish

Depositor

Daniel Hesselson, Didier Stainier

Article

Hesselson et al Curr Biol. 2011 Apr 26;21(8):712-7. doi:

Inserts

Cre
Venus

Use

Cre/Lox; Zebrafish transgenesis

Tags

mCherry

Promoter

Crystalin and Elastase

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA-AAVS1_hspCas9

Plasmid

#193309

Purpose

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a SpyCatcher-SnoopCatcher

Plasmid

#72324

Purpose

Bacterial expression of SpyCatcher linked to SnoopCatcher.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SpyCatcher-SnoopCatcher

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

35 amino acids at the N-term of SpyCatcher have b…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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