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Showing: 2061 - 2080 of 2760 results

  1. pJK377

    Plasmid
    #72411
    Purpose
    Produces Acetobacter aceti 1023 Est05
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72411-plasmid-map-sequence-id-135530

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  2. ORFtag_TetR-SD_Frame2

    Plasmid
    #220982
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220982-plasmid-map-sequence-id-437118

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  3. ORFtag_TetR-SD_Frame3

    Plasmid
    #220983
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220983-plasmid-map-sequence-id-437500

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  4. pNHA_OROV_NCP

    Plasmid
    #208937
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Nucleocapsid
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted start codon so HA included at N-terminus
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208937-plasmid-map-sequence-id-418963

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  5. pJK406

    Plasmid
    #72420
    Purpose
    Produces Acetobacter aceti 1023 Est05, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…
    Promoter
    T7
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72420-plasmid-map-sequence-id-135533

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  6. pJK267

    Plasmid
    #72432
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    T. J. Kappock
    Insert
    Acetobacter aceti 1023 gDNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    n/a
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pJK368

    Plasmid
    #72407
    Purpose
    Produces Acetobacter aceti 1023 Est12
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72407-plasmid-map-sequence-id-135653

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  8. m2ap

    Plasmid
    #50051
    Purpose
    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
    Depositor
    Timothy Springer
    Insert
    M2AP
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    starts at aa47; N127R, S158A, S177N, N357S, N463S…
    Promoter
    CMV
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. ORFtag_TetR-SD_Frame1

    Plasmid
    #220981
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220981-plasmid-map-sequence-id-437540

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  10. Mek2

    Plasmid
    #40776
    Depositor
    Dustin Maly
    Insert
    MKK2 (MAP2K2 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Starting Met removed
    Promoter
    CMV
    Available since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only

  11. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  12. Unc104_K560GFP

    Plasmid
    #129773
    Purpose
    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    Unc104 (unc-104 Nematode)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 361, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129773-plasmid-map-sequence-id-263568

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  13. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  14. pJK404

    Plasmid
    #72417
    Purpose
    Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72417-plasmid-map-sequence-id-135656

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  15. Mek1

    Plasmid
    #40774
    Depositor
    Dustin Maly
    Insert
    MKK1 (MAP2K1 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Starting Met removed
    Promoter
    CMV
    Available since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only

  16. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid
    #41878
    Depositor
    Anna Huttenlocher
    Insert
    photoactivatable Rac1 (RAC1 Human)
    Use
    Zebrafish expression
    Tags
    6x His and mCherry
    Expression
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    Available since
    Oct. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    41878-plasmid-map-sequence-id-56671

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  18. pENTR223 TMPRSS2, closed

    Plasmid
    #149721
    Purpose
    for Gateway cloning of TMPRSS2 into destination vector
    Depositor
    Anne-Claude Gingras
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Entry vector
    Tags
    Expression
    Mutation
    wt, without start codon
    Promoter
    Available since
    June 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149721-plasmid-map-sequence-id-298609

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  19. pSecUAG_UTu1D

    Plasmid
    #233867
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant
    Depositor
    Dieter Söll, Natalie Krahn
    Inserts
    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG) and …
    Promoter
    EM7, araBAD, and native As SelD
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233867-plasmid-map-sequence-id-471093

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  20. pKAN43Ag8-GFP

    Plasmid
    #178182
    Purpose
    Plant binary vector expressing GFP by a enhanced 35S promoter
    Depositor
    Keiichi Shimizu
    Insert
    EGFP (eGFP )
    Use
    Tags
    Expression
    Plant
    Mutation
    The CAT is inserted after the start codon.
    Promoter
    The minimal 35S promoter from Cauliflower Mosaic …
    Available since
    Sept. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    178182-plasmid-map-sequence-id-446882

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Showing: 2061 - 2080 of 2760 results