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Plasmid#164210PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoterDepositorAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only
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pMOD_C2200
Plasmid#91082PurposeModule C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35SDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRPromoternone (will be fused to gRNA array in MODULE B)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PDHA1-N-10
Plasmid#58195PurposeLocalization: Mitochondria, Excitation: N/A, Emission: N/ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPDHA1 (PDHA1 Human)
TagsSNAP tagExpressionMammalianPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCFD5-NS
Plasmid#149546PurposeExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgLAP3
Plasmid#19704DepositorInsertpgLAP3
TagsEGFP, S peptide, and TEVExpressionMammalianAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mito-mGreenLantern
Plasmid#164461PurposeLocalization of mGreenLantern fluorescent protein to mitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmGreenLantern
TagsHuman COX8a first 29 amino acidsExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Plasmid#62373PurposeExpression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cellsDepositorInsert(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Tags6xHis tag, (-30)dGFP, Cathepsin cleavage site, an…ExpressionBacterialMutationY67S amino acid substitution in (-30)GFP renders …PromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_003
Plasmid#192505PurposeFor cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFPDepositorInserthU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
UseLentiviralPromoterhU6Available sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP scFv [N86/38]
Plasmid#182095PurposeMammalian Expression of GFP scFv. Derived from hybridoma N86/38.DepositorInsertrecombinant mouse scFv targeting GFP
TagsHA, HisExpressionMammalianPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
S1m tagged DANCR
Plasmid#138585PurposeFor in vivo taggingDepositorAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet_NADP-Snifit-mito
Plasmid#113919PurposePlasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNADP-Snifit
ExpressionMammalianPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
GUT BuGZ FL
Plasmid#84024PurposeExpression of GFP tagged BuGZ in human cellsDepositorInsertBuGZ (ZNF207 Human)
UseLentiviralTagsTurboGFPExpressionMammalianMutationQ394RPromoterUCOE / SFFVAvailable sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEP179_pX330K
Plasmid#137882PurposeEntry vector for multi-gRNA cloningDepositorTypeEmpty backboneExpressionMammalianAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV2-GCaMP6f-P2A-H2B-PAmCherry
Plasmid#133419PurposeFluorescent reporter for calcium imaging and photoactivationDepositorAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHD-DsRed-attP-w+
Plasmid#80898PurposeVector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.DepositorInsertsattP
LoxP-3xP3-DsRed-LoxP
GMR-white
UseHigh copy, amp resistancePromoter3xP3 and GMR::hsp70Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-FIP3
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialPromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-TFR-20
Plasmid#56488PurposeLocalization: Transferrin Receptor, Excitation: 485, Emission: 507DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTFR (TFRC Human)
TagssfGFPExpressionMammalianPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDRM209 LTP (Luciferase tdTomato Puro)
Plasmid#174723PurposeExpresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
pac
UseLentiviral and LuciferaseTagsE2A linker and T2A linkerExpressionMammalianPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet
Plasmid#125134PurposeNatively produces mNeon, in the presence of Cre will be recombined to produce mScarlet.DepositorTypeEmpty backboneExpressionMammalianMutationRemoved Not1 site in mScarletAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by