We narrowed to 5,929 results for: pcas
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Plasmid#103862PurposeExpresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianAvailable SinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
CROPseq-EFS-HypaSpCas9-P2A-EGFP
Plasmid#106964Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralExpressionMammalianPromoterEFSAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-tdTomato-WPRE
Plasmid#51503PurposeCan be used to generate AAV virus that will express tdTomato in the presence of CreDepositorInserttdTomato
UseAAVPromoterpCAGAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
peSpCas9(1.1)-2×sgRNA (IFT88, donor)
Plasmid#80769PurposeExpresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).DepositorAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-HypaSpCas9-P2A-puro
Plasmid#106965Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralExpressionMammalianPromoterEFSAvailable SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV1 for Split-PE, SpCas9 nickase (PKC1001)
Plasmid#190111PurposeAAV1 for expression of nickase SpCas9(H840A) for Split-PE useDepositorInsertnSpCas9
UseAAVTagsbpNLSMutationH840APromoterEF1aAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE
Plasmid#51502PurposeCan be used to generate AAV virus that will express EGFP in the presence of CreDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVPromoterCAGAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9(D10A)-T2A-EGFP
Plasmid#221230PurposeExpress nCas9 nickase (D10) with GFP reporterDepositorInsertSpCas9(D10A)-T2A-EGFP
ExpressionMammalianMutationD10AAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-EYFP-WPRE-bGHpA
Plasmid#213971PurposeAAV expression of EYFP from pCALM1 promoterDepositorInsertpCALM1
UseAAVAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4 GlyR IRES EGFP
Plasmid#119739PurposeChemogenetic inhibitor expression plasmidDepositorInsertPSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
ExpressionMammalianMutationL131G, Q139L, Y217FPromoterCAGAvailable SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9.EF1a-BFP.sgLMNA
Plasmid#98971PurposeCas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.DepositorInsertLMNA sgRNA and spCas9
ExpressionMammalianAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GPHN-FingR-mGold2s-CCR5TC
Plasmid#231785PurposeVisualization of inhibitory post-synapse using GPHN-FingR-mGold2sDepositorInsertGPHN-FingR-mGold2s-CCR5TC
ExpressionMammalianPromoterActin promoter with CMV enhancerAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 Alpha Spike
Plasmid#185691PurposeExpresses codon-optimized full length SARS-CoV-2 Alpha SpikeDepositorInsertSARS-CoV-2 Alpha Spike (S )
ExpressionMammalianMutationContains the following mutations: Δ69-70, Δ145, N…Promoterchicken β-actin promoter, CMV enhancerAvailable SinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-NLS-dPspCas13b-3xmRuby3-NLS
Plasmid#132405Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralExpressionMammalianMutationH133A; H1059AAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG001 His6-TwinStrep-SUMO-AapCas12b
Plasmid#153162PurposeBacterial expression plasmid for AapCas12bDepositorInsertAapCas12b
ExpressionBacterialAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MCP-dNS-SRRM1-V5-mCherry
Plasmid#235095PurposeSRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domainDepositorInsertpCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)
ExpressionMammalianMutationThe SRRM1 entry clone from DNASU was modified usi…PromoterCAGAvailable SinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4 5HT3 LC IRES GFP
Plasmid#123066PurposeChemogenetic activator expression plasmidDepositorInsertPSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
ExpressionMammalianMutationL131G Q139L Y217FPromoterCAGAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-470)
Plasmid#170536PurposeExpresses PTPIP51(1-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
TagsFLAG tagExpressionMammalianPromoterCAG promoterAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0043-PspCas13b-gRNA non target
Plasmid#223698PurposegRNA control for dPspCas13b-FTODepositorInsertgRNA target sequence for luciferase control
UseCRISPRExpressionMammalianAvailable SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS2-TEV-Twin-Strep
Plasmid#202529PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cellDepositorInsertSGMS2 (SGMS2 Human)
TagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TGA)PromoterCAG and chicken β-actin promoterAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only